Relationship Between Mutation Of Kras Gene And Clinical Pathobiological Characteristics In Colorectal Cancer

ObjectiveIn this study,the detection of Kras gene in colorectal cancer tissues was used to investigate the mutation of Kras gene in colorectal cancer tissues,the relationship between the mutation status of Kras gene and clinicopathological characteristics and prognosis of patients,and provide reference for clinical treatment.MethodsFrom the First Affiliated Hospital of Nanchang University,from July 2017 to January 2019,patients with colorectal cancer were diagnosed with preoperative informed consent and voluntary genetic testing in 29 cases.NGS sequencing was used to detect mutation of Kras gene in colorectal cancer tissues.The relationship between different mutations and gender,age,tumor markers,presence or absence of neurovascular infiltration,lymph node metastasis,tumor location,degree of differentiation,and postoperative pathological staging were compared.Results(1)The mutation rate of Kras gene in colorectal cancer was 34.48%.The main mutation types were 12 codon mutations(GGT?GAT,GGT?GTT,GGT?TGT)and 13 codon mutations(GGC?GAC),the 12 and 13 codons were not found to be mutated at the same time.(2)Kras gene mutation was not associated with gender,age,tumor markers,lymph node metastasis,neural vessel infiltration,tumor location,degree of differentiation and postoperative pathological stage in patients with colorectal cancer.(3)Mutations in the Kras gene are associated with poor prognosis in patients with colorectal cancer.Conclusions(1)The mutation rate of Kras gene in colorectal cancer is high,and it is necessary to routinely perform Kras gene detection in patients with colorectal cancer.(2)The Kras gene mutation has nothing to do with the clinicopathological features of patients with colorectal cancer.(3)The Kras gene mutation has a significant correlation with the prognosis of patients with colorectal cancer.