The Clinical Significance Of C-met Protein Expression And KRAS,PIK3CA Gene Mutation In Primary Colorectal Cancer And Liver Metastases

Objective:To investigate the expression of C-met protein and the state of KRAS and PIK3CA gene mutation in primary tumor and corresponding liver metastases in colorectal cancer(CRC),and combined analyze its correlation with clinicopathological parameters and prognosis of CRC.Methods:1.To detect the expression of C-met proteins about 58 cases in primary lesions of colorectal cancer(CRC)and corresponding liver metastasis tissue by immunohistochemical staining,to analyze the differences of C-met protein expression between two groups.2.To detect the gene mutation status of KRAS and PIK3CA about 58 cases in primary lesions of colorectal cancer(CRC)and corresponding liver metastasis tissue by Real-time PCR.Results:1.The high expression rates of C-met protein were 74.14%(43/58),81.03%(47/58)respectively in primary lesions of colorectal cancer and corresponding metastases tissue.The high expression of C-met in primary lesion was associated with clinical stage,the high expression of C-met in metastasis was associated with primary lesion.2.The mutation rates of KRAS were 31.03%(18/58),25.86%(15/58)respectively in primary lesions of colorectal cancer and corresponding metastases tissue,and G12 D wasthe most common mutation site.The mutation rates of PIK3CA were 8.62%(5/58),10.34%(6/58)respectively,in which the most common mutation site was E545 K.Only one case occurred simultaneously both of KRAS and PIK3CA.3.KRAS and PIK3CA gene mutation has a good consistency of the primary lesions of CRC and corresponding liver metastasis(Kappa?0.75).4.Synchronous liver metastasis and the mutation of KRAS are risk factors about prognosis of CRC.Conclusion:1.The high expression of C-met in primary lesion was associated with clinical stage,the high expression of C-met in liver metastasis was associated with primary lesion.2.The mutation of KRAS was related to the primary lesion of tumor location,the quantity of metastasis and the tupes of tumor.The mutation of PIK3CA was associated with Synchronous / metachronous liver metastasis and the quantity of metastasis.3.Primary lesions /corresponding liver metastasis can be as the source of molecular detection of the specimen.4.Simultaneous liver metastases and the mutation of KRAS were associated with poor prognosis of CRC.