| Objective: Low back pain is a common type of disease in spinal diseases.Lumbar disc herniation(LDH)is the most common disease,which is considered to be the main cause of intervertebral disc degeneration(IVDD).It has a great impact on the lives and economics of patients.The pathogenesis of intervertebral disc degeneration has not yet been fully elucidated,and the stability of the nutrition and metabolism of the intervertebral disc plays an important role in the degeneration of the intervertebral disc.It has been found that connexin 43(Cx43)plays an important role in maintaining the stability of nutrition and metabolism of bone and chondrocytes.In this study,according to the magnetic resonance imaging(MRI)Pfirrmann and pathological grading of lumbar disc degeneration,the expression of Cx43 in the intervertebral disc tissues of various degraded grades was detected by immunohistochemistry and Western blot,and to observe the correlation between Cx43 and intervertebral disc degeneration.It is expected to prove that Cx43 has a significant correlation with Lumbar disc degeneration.Methods: 23 patients who met the criteria were included in the experimental group,and 5 patients with lumbar fractures due to trauma were included in the control group.The endplates and nucleus pulposus tissues harvested from the patient were collected.Firstly,the degeneration of intervertebral disc tissue was observed by Hematoxylin-eosin(HE)staining and classified according to pathological Thompson intervertebral disc degeneration grade and imaging Pfirrmann grading.Secondly,immunohistochemistry and Western Blot were used to detect the expression of Cx43 in the endplate and nucleus pulposus of 28 patients in each group,and finally the measured data were statistically analyzed by spss23.0 software.The difference between the experimental group and the control group was analyzed by two independent sample t-tests.Correlation between the expression of Cx43 in the intervertebral disc and the degree of disc degeneration was analyzed by spearman rank correlation coefficient.Differences in the degree of disc degeneration between the two grades of pathology and imaging were analyzed by paired t-test.Results: 1.The WB of the control group was 0.887±0.147,the experimental group was 0.486±0.132,t=6.048,p=0.000<0.05.Therefore,the WB content of the experimental group and the control group was statistically different.The WB content of the control group was significantly higher than that of the experimental group.The average IHC control group was 0.191±0.056,the experimental group was 0.055±0.031,t=7.635,p=0.000<0.05.Therefore,the IHC content in the experimental group and the control group was statistically different.The IHC content in the control group was significantly higher than that in the experimental group.2.The correlation coefficient between pathological grade and WB was R=-0.931,p=0.000<0.05;the correlation coefficient with IHC was R=-0.931,p=0.000<0.05,the correlation was statistically significant,and the correlation coefficient was large.There is a high negative correlation,that is,the higher the pathological grade,the lower the expression of WB and IHC.3.Using the paired t test for differential analysis,the results showed that t=-3.102,p=0.005,so the imaging grading was different from the pathological grade,the difference was statistically significant,and the pathological grade was higher than the imaging grade 0.3 Units.Conclusion: 1.Cx43 is significantly associated with Lumbar Disc Degeneration.The expression of Cx43 in normal intervertebral disc is significantly higher than that in degenerative intervertebral disc,and the higher the degree of intervertebral disc degeneration,the lower the expression of Cx43,indicating that Cx43 is involved in the process of intervertebral disc degeneration.2.Imaging grade and pathological grade have different degrees of grade disc degeneration,and pathological grade may be more accurate.3.Cx43 may be a new direction to study the early prevention and treatment of intervertebral disc degeneration,thereby reducing the incidence of lumbar disc herniation. |
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