Effects Of Smoking On The Expression Of IL-4 And IL-17 In Induced Sputum Of Patients With Lower Respiratory Infection

ObjectiveTo explore the effect of smoking on the expression of IL-4 and IL-17 in induced sputum of lower respiratory tract infections,and to explore the mechanism of tobacco smoke stimulating the human inflammatory response.To compare the effects of smoking on the expression of IL-4 and IL-17 in induced sputum of lower respiratory tract infections and normal adults.The Th2 cell plays an important role in the mechanism.At present,there is evidence that IL-17 can participate in the immune response,which can destroy the balance of the human immune defense system,and eventually lead to systemic immune inflammatory disease.Therefore,we observed changes in induced sputum levels of IL-4 and IL-17 in smoking and non-smoking respiratory infections and normal physical examinations to explore the effect of smoking on lower respiratory tract infection and analyze the effect of smoking on the respiratory microecological imbalance in patients.MethodsOne hundred and twelve patients with lower respiratory tract infection were selected from January 2017 to March 2018 in the Department of Respiratory Medicine of the Second Affiliated Hospital of Shenyang Medical College.According to symptoms,signs and auxiliary examinations,they were divided into community-acquired pneumonia group?27 smokers,31 non-smokers?,acute attack stage of chronic bronchitis group?26 smokers,28 non-smokers?and selected at the same time in our hospital.There were 42 cases in the physical examination group?22 smokers and 20 non-smokers?.The smoking group with lower respiratory tract infection was divided into infected smoke SI<400 subgroup,infected smoking SI?400 subgroup.General data of patients?age,sex and so on?were collected.The expression levels of IL-14 and IL-17 in induced sputum were detected by ELISA,and the results were analyzed.ResultsIn the community acquired pneumonia group,compared with non-smokers,the expression levels of IL-4 and IL-17 in induced sputum of smokers increased significantly?P<0.05?.In acute attack stage of chronic bronchitis group,compared with non-smokers,the expression levels of IL-4 and IL-17 in induced sputum of smokers increased significantly?P<0.05?.Compared with non-smokers,the expression of IL-4 and IL-17 in induced sputum of smokers increased?P<0.05?.In the smoking subgroup of lower respiratory tract infection,compared with non-smokers and mild smoking subgroup,the expressions of IL-4 and IL-17 were increased in severe smoking subgroup?P<0₀5?.Compared with non-smokers,the expressions of IL-4 and IL-17 were increased in mild smoking subgroup?P<0.05?.Conclusions1.Smoking can affect the expression le'vels of IL-4 and IL-17 in sputum induced by lower respiratory tract infection.Cigarette smoking increases the levels of IL-4 and IL-17 of the lower respiratory tract infections?social acquired pneumonia and acute attack of chronic bronchitis?.2.According to the difference of smoking index?branch year?,smokers with lower respiratory tract infections?social acquired pneumonia and acute attack of chronic bronchitis?were divided into mild smoking subgroup?SI<400?and severe smoking subgroup?SI?400).The test showed that the expression of IL-4 and IL-17 was higher in the severe smoking subgroup than mild smoking subgroup and non-smokers,the expression of IL-4 and IL-17 was higher in mild smoking subgroup than non-smokers.