Effects Of Smoking On The Expression Of IL-4,IL-5,IL-17,IFN-? And CD4+ In The Serum Of Patients With Respiratory Infection

ObjectiveTo explore the effect of smoking on the expression of L-4,IL-5,IL-17.IFN-y and CD4+ in the serum of respiratory tract infections,and to explore the mechanism of tobacco smoke stimulating the human inflammatory response.To compare the effects of smoking on the expression of IL-4,IL-5,IL-17,IFN-? and CD4+ in the serum of respiratory tract infections and normal adults.The smoking index can indicate the degree of human exposure to tobacco smoke and explore the relationship between the amount of smoke exposure and the severity of the inflammatory response.The Th1/Th2 cell imbalance theory plays an important role in the mechanism.At present,there is evidence that IL-17 can participate in the immulne response,which can destroy the balance of the human immune defense system.and eventually lead to systemic immune inflammatoiy disease[1].Therefore,we observed changes in the serum levels of IL-4.IL-5,IL-17.IFN-?,and CD4+ in smoking and non-smoking respiratory infections and normal physical examinations.4.The changes of IL.-5,IL-17.IFN-? and CD4+expression were analyzed to analyze the effect of smoking on the respiratory microecological imbalance in patients.MethodsSelected 101 patients with respiratoiy infection(smoking and non-smoking)from March 2016 to March 2017 in the Department of Respiratory Medicine of the Second Affiliated Hospital of Shenyang Medical College;selected from March 2016 to March 2017 Physical examination(smoking and non-smoking)100 cases,Divided into infected smoking group,infected non-smoking group,healthy smoking group and healthy non-smoking group,according to the smoking index(year)is divided into infected smoke SI<400 subgroup,infected smoking SI ? 400 subgroup;healthy smoking SI<400 Subgroup,healthy smoking SI? 400 subgroup.The levels of IL-4.IL-5,IL-17.IFN-? and CD4+ in serum were detected by enzyme-linked immunosorbent assay(ELISA).Independent sample T test was used.Count data were compared with x2 test.One-way analysis of variance was used to compare the mean,P<0.05 was considered statistically significant.ResultsCompared with the non-smoking group,smoking increased white blood cells,increased the percentage of neutrophils,decreased the percentage of lymphocytes,increased C-reactive protein,and increased procalcitonin(P<0.01).Smoking can make patients with respiratory infections The severity of consolidation or interstitial edema increased in chest imaging,and the number of patients with pleural effusion increased(P<0.01).The levels of IFN-? and CD4+ in the infected group increased(P<0.05),and the expression of IL-5 decreased significantly(P<0.01).The levels of IL-17 and IFN-? in the healthy smoking group were significantly lower than those in the healthy non-smoking group(P<0.05).Among the subgroups of infected smokers,the levels of IL-4,IL-5,and CD4+ in the SI<400 smoking group were significantly higher than those in the SI?400 smoking group(P<0.01);IL-17 was decreased(P<0.05)and IFN was higher in the SI group.-? decreased significantly(P<0.01).IL-4,IFN-?,and IL-17 levels in the SI<400 smoking group were significantly lower than those in the non-smoking group(P<0.01).and CD4+ levels were higher than those in the non-smoking group(P<0.05).IL-4 was decreased in patients with SI ?400 smoking(P<0.05);IL-5 and IL-17 were significantly lower than those in the non-smoking group(P<0.01);CD4+ was significantly increased compared with the non-smoking group(P<0.01).Among the subgroups of healthy smoking,IL-4,IL-5,IFN-?,IL-17,and CD4+ in the SI<400 smoking group were significantly higher than those in the SI ?400 smoking group(P<0.01).IL-5 and IFN-? in patients with SI<400 smoking group were significantly lower than those in healthy non-smoking group(P<0.01).IL-4,IL-5,IFN-?,and IL-17 in the SI ?400 smoking group were significantly higher than those in the healthy non-smoking group(P<0.01).Conclusions1.Cigarette smoking reduces the levels of IL-17 and IFN-? in the blood of respiratory infections and increases the levels of IL-4 and IL-5.Through the imbalance of Thl/Th2 system imbalance,affect the occurrence of infectious diseases.2.According to smoking degree,smoking was divided into mild smoking subgroup(SI<400)and severe smoking subgroup(SI ? 400).The test showed that IL-17 and IFN-? expression was inhibited in the mild smoking subgroup and the expression of IL-4 and IL-5 was inhibited;IL-17,IFN-? and IL were compared between the severe smoking subgroup and the mild smoking subgroup.-4,IL-5,CD4+expression increased significantly.