| Objective:To study the feasibility of tree shrews as an Epstein-Barr virus-associated human disease model by investigating the EB virus in different age groups.Methods:Eight tree shrews of 2 months old,4 months old,and 12 months old were selected from the experimental group.500 ul of virus suspension containing 2.1×10~8 copies of EBV was inoculated by oral and nasal combination.Four blank 12-month tree shrews were selected in the blank group.Oral administration of 500 ul of RPMI 1640 medium.After inoculation,the body temperature,body weight and femoral vein blood were collected regularly.The expression of EBV-related genes was detected by RT-PCR.The changes of serum EBV-related antibodies were detected by ELISA.The dead tree shrews were dissected,HEhistopathologicalchangeswereobserved,and immunohistochemistry was performed.Expression of LMP1 and EBNA1proteins in various tissues.RESULTS:The temperature of the EBV virus in different age groups increased after the infection of EBV virus.The tree shrews were most obvious at 4 months old,and the transient weight loss was observed in the 4 months old tree shrew.The 12-month-old and 4-month-old tree shrews were After the vaccination,the number of white blood cells increased and the percentage of lymphocytes increased.At the same time,three animals were observed to be similar to human acute mononucleosis,and one had EBV-associated hematopoietic syndrome.Conclusion:Different ages of tree mites infected with EBV have different characteristics and can induce EBV-related diseases.Tree shrews have potential as an animal model of EBV-related diseases. |
