| EBV(Epstein-Barr virus) is discovered by Epstein and Barr in1964, and it is the first time successfully obtained by suspension culture in vitro with lymphoma cells of Burkitt African children. EBV has a prevalent infection in the world, more than90%people infected by EBV. EBV mainly spreads through saliva and blood infection. Most infections occurred in childhood, it is not accompanied or only with mild clinical symptoms. EBV infection can induce a variety of malignant tumors which including Burkitt lymphoma, gastric cancer, nasopharyngeal carcinoma and Hodgkin lymphoma, is also often associated with other viral infections. There has no effective treatment and drugs to prevent or cure EBV infection currently. To find the treatment of EBV associated diseases and do more thoroughly studies of the EBV, a kind of animal model which is easy to be studied is essential. So far many animal models of EBV have been reported with just a few primates have susceptibility to EBV. Unfortunately the number of the primate animal models is drastically reduced and the high price is not suitable in general experiments.The study is to explore the sensitivity of EBV infection on guinea pigs(Hartley), in order to find theory support in the research of EBV animal models. Guinea pig is a common experimental animal which has been widely used in researching infectious diseases and is susceptibility to varieties kinds of virus. Guinea pig has many advantages, such as, easy to be raised; gently temperament; small body type; easy to manipulating in experiments. The experiment method is that10guinea pigs were immuned with EBV by both nasal and oral inoculation; another10guinea pigs were immuned by intravenous inoculation. There are three aspects to evaluate the susceptibility of EBV on guinea pigs, which are viral antigens expression, specific antibody titers in serum and pathological analysis of guinea pigs’ organs and tissues. The results showed that six of ten experimental guinea pigs which were immuned with both nasal and oral inoculation were detected antigen of BcLF1expression and four in the six guinea pigs were detected antigen of EBNA1expression; the antibodies of ten guinea pigs are universally increased after infected; livers, lungs, spleens, parotid glands of five guinea pigs in experimental group guinea pigs are detected inordinately lesion by pathological analysis. Another group of the10guinea pigs with intravenous inoculation did not detect antigens of antigens of BcLFl and EBNA1expression; but the antibodies of10guinea pigs are also universally increased after infected; livers are detected light lesion by pathological analysis. All results come to the conclusion that guinea pigs can be used as alternative animal model for researching Epstein-Barr virus. |
