Research On The Association Of Rs1051931 And Rs1805017 SNPs With Susceptibility,Risk Factors And Clinical Feature Of Acute Myocardial Infarction In Guangxi Han Population

BACKROUND: Accompany with the rapid development of economy in our country,quality of resident's life has been continuously improved and their pace of life has been accelerated.Cardiovascular disease has become the leading cause of death in China,accounting for 40% of the total mortality.Among them,acute myocardial infarction(AMI)is one of the most common and most serious types of cardiovascular diseases.The early prediction and prevention of AMI is increasingly important for human health problems.Single nucleotide polymorphisms(SNPs)are a group of polymorphisms that are of interest in genetic polymorphisms.That is,a single nucleotide is missing,inserted,or replaced on the same DNA allelic sequence.The conversion to other nucleotides has been found to exceed 9 million SNPs,accounting for more than 90% of genetic polymorphisms.SNPs are considered to be important influencing factors that exhibit different susceptibility to different individuals for the same disease or the same environmental factors.With the maturation and development of gene sequencing technology,more and more pathogenic susceptibility loci have been found.SNPs of the PLA2G7 gene located in the human chromosome 6p12.3 have been shown to be associated with the development of acute myocardial infarction(AMI)and are encoded by the PLA2G7 gene.Lipoprotein-associated phospholipase A2(Lp-PLA2)increases the risk of acute myocardial infarction by promoting the formation and development of atherosclerosis.At present,studies on the association between rs1051931 and rs1805017 SNPs on PLA2G7 gene and AMI have rarely been reported.In particular,there is a lack of correlation between PLA2G7 gene rs1051931 and rs1805017 SNPs and clinical features of AMI.Association between rs1051931 and rs1805017 SNPs on PLA2G7 gene and AMI have rarely been reported.In particular,there is a lack of correlation between PLA2G7 gene rs1051931 and rs1805017 SNPs and clinical features of AMI.Therefore,studies on the association of PLA2G7 gene rs1051931 and rs1805017 SNPs with the risk of AMI are beneficial for us understanding the genetic characteristics and AMI-related genes,provide scientific evidence for the screening of high-risk AMI patients and promote the development of AMI prevention and early intervention.OBJECTIVES: To study the association of rs1051931 and rs1805017 snps with susceptibility,risk factors and clinical feature of acute myocardial infarction in guangxi Han population.METHODS: In 315 Chinese Han AMI patients and 334 Chinese Han healthy subjects at the First Affiliated Hospital of Guangxi Medical University between December 2012 and December 2016,PLA2G7 rs1051931 SNPs and rs1805017 SNPs were performed using PCR-RFLP.Blood lipid levels were measured,BMI was calculated based on height and weight.Some possible cardiovascular risk factors(gender,age,family history,past history,tobacco and alcohol consumption,etc.)were collected by our epidemiological investigation.Test group and control group were compared the discrepancy of genotypic and allelic frequencies,so as to identify the relationship between two PLA2G7 SNPs(rs1051931 and rs1805017)SNPs and susceptibility for AMI.The potential risk factors of AMI were determind by Non-conditional binary logistic regression analysis.RESULTS: 1.The values of mean age(61.56 ± 10.73 vs.57.63 ± 10.93)and BMI(23.85 ± 3.22 vs.22.45 ± 3.11)were higher in the AMI group than the control group.The percentages of those who smoked and consumed alcohol were 157(49.84%)and 93(29.52%)in the AMI group,respectively,and 81(24.25%)and 63(18.86%)in the control group,respectively.Significantly more subjects in the AMI group smoked and consumed alcohol than in the control group(p < 0.05 for each).There were no significant difference in sex ratio between the two groups(p > 0.05).The levels of TC,TG and LDL-C were 4.53 ± 1.24 mmol/L,1.66±1.11 mmol/L and 2.98 ± 0.88 mmol/L in the test group,respectively,and 4.30 ± 0.53 mmol/L,1.39±1.06 mmol/L and 2.55 ± 0.51 mmol/L in the control group,respectively.The levels of serum TC,TG and LDL-C in the AMI group were higher than those in the control group(P < 0.05 for each).However,the serum HDL-C level of control group was higher than AMI group(1.71 ± 0.39mmol/L vs 1.12 ± 0.33 mmol/L,P < 0.05).2.The GG genotype and G allele frequency,respectively,were 73.65% and 86.19% in the AMI group,which was lower than those in the control group,82.63% and 91.17%.The frequencies of the GA+ AA genotypes and the minor A allele were 26.35% and 13.81%,respectively,in the AMI group,which was significantly higher than the 17.37% and 8.83% in control group.Obvious discrepancies in both genotypic and allelic frequencies between test and control groups were explored(P < 0.025 for each).PLA2G7 rs1805017 SNPs: In AMI and normal group,the frequencies of AA,GA and GG genotype respectively were 3.49%?31.75%?64.76% and 1.81%?31.74%?66.47%;G and A allele frequencies respectively were 80.63%?19.37% and 82.34%?17.66%;There were no significant statistical differences in genotypic and allelic frequencies between these two groups(P > 0.025 for each).3.Non-conditional binary logistic regression analysis showed that diabetes,high blood pressure,smoking,age,LDL-C,BMI,rs1051931 and sex were strongly associated with AMI risk,with OR values of 92.736,4.056,2.825,2.061,3.109,1.671,1.954 and 2.080 respectively.In contrast,HDL-C was negatively correlated with the risk of AMI,with an OR value of 0.028(P < 0.025 for each).However,no significant differences were seen between AMI and control groups in terms of correlation of TC,TG and alcohol consumption with the risk of AMI(P > 0.025 for each).CONCLUSIONS: 1.The data in the present study indicated that the GA and AA genotypes and minor A allele of the PLA2G7 rs1051931 SNPs were positively correlated with the risk of AMI.There were no significant statistical differences in genotypic and allelic frequencies in PLA2G7 rs1805017 SNPs Loci between the two groups.2.It was verified again that diabetes,high blood pressure,smoking,age,LDL-C,BMI,rs1051931 and sex were risk factors for AMI,while HDL-C was negatively correlated with AMI risk.3.In our study,there were not any powerful evidences to support a relationship of PLA2G7 rs1051931 and rs1051931 SNPs with typical symptoms,infarction location,diagnosis time and serious complications.