The Relationship Between MYO9B Gene Polymorphism And Inflammatory Bowel Disease In Guangxi Zhuang Population

Background:Inflammatory bowel disease(IBD)is a group of immune-mediated chronic non-specific inflammatory bowel diseases,mainly divided into ulcerative colitis(UC)and crohn's disease(CD),which are affected by environmental factors,genetic factors,immune status and other aspects.Genome-wide association analysis(GWAS)studies have shown that some genetic susceptibility genes associated with intestinal barrier function,inflammation,and immune response can promote the development of IBD.Studies at home and abroad have found that MYO9 B gene mutation is related to the incidence of IBD,but the conclusions obtained from different regions and populations of different ethnic groups are slightly different,and the existence of gene polymorphism is affected by many aspects.Objective:Analyze the relationship between MYO9 B gene rs962917 and IBD population in guangxi zhuang nationality.Methods:Firstly,The intestinal mucosa tissues of 153 IBD patients(only han and zhuang patients)in guangxi zhuang autonomous region were selected as the case group,and the intestinal mucosa tissues of 155 healthy subjects(only han and zhuang healthy subjects)in the same region were selected as the controlgroup.Secondly,by extracting DNA from intestinal mucosa tissues of each experimental group to the gene extraction kit,the MYO9 B gene target fragment containing SNP site rs962917 was designed.Finally,PCR products were obtained by PCR amplification,and then analyzed and compared by the sequencing results.Results:Comparing the genotype frequency of MYO9 B SNP site rs962917 between the CD group and the control group of zhuang and han nationality,the P values were < 0.05,and the difference was statistics significance.Comparing the genotype frequency of MYO9 B rs962917 between the zhuang and han groups,the P values were< 0.05,and the difference was statistics significance.Conclusion:MYO9B rs962917 is related to CD susceptibility in guangxi zhuang nationality,and MYO9 B rs962917 gene polymorphism is related to CD incidence in guangxi zhuang nationality.