Expression And Clinical Significance Of Slit2 In Bladder Tumors

PurposesBladder cancer is a common malignant tumor in the clinic,and its etiology has not been studied yet.At present,the treatment is mainly based on early diagnosis and surgical resection.The study of genes and signaling pathways can help early diagnosis of bladder cancer,help early treatment of bladder cancer patients and improve prognosis.Current research has found that Slit/Robo signaling pathway regulates tumor cell metastasis and tumor angiogenesis.To date,these studies have shown that the Slit/Robo signaling pathway is likely to be an important signaling pathway for tumorigenesis and metastasis;current studies have confirmed that the Slit/Robo pathway primarily affects advanced cancer.The Slit/Robo pathway interacts with breast cancer E-cadherin and ?-catenin and colorectal cancer to inhibit cell invasion.While Slit2/Robo1 specifically inhibits hepatocyte growth factor(HGF)-mediated cell migration in liver cancer.HGF is associated with its ligands,and most studies have shown that Slit2 primarily inhibits cancer cell invasion and migration.Denk et al.found that Slit3 also inhibits cell migration of melanoma cells by modulating activator protein-1(AP-1).In addition to prostate cancer and colorectal cancer,Slit/Robo inhibits tumors by inhibiting cell invasion and migration.It is unclear why differences occur in different tumors.One possible reason may be that Slit2 binds to the Robo1 receptor more specifically than Slit1 or Slit3 in the three Slits.Therefore,we consider whether Slit2 is involved in the pathogenesis of bladder cancer;for this reason,we conducted related research,and previous studies suggest that Robo1 and Robo4 may be involved in inhibiting bladder cancer angiogenesis and inhibiting bladder cancer formation,thereby inhibiting the growth of bladder tumor cells.In view of the literature search,the results of Slit2 gene and bladder cancer pathology are very few.Therefore,in order to reveal the pathology of bladder cancer and to find key molecular markers,we have examined the pathological specimens of bladder cancer patients and applied biological techniques.To study the expression of Slit2 gene in tumor cells,to reveal the effect of Slit2 gene on the division and growth of bladder cancer cells,and to find new methods and targets for the treatment of bladder cancer.Materials and Methods1 Materials 1.1 Specimens: From October 2016 to December 2018,43 patients with bladder cancer who underwent surgical treatment in the Department of Urology,Huaihe Hospital,Henan University.And some other form zhe First Affiliated Hospital of Zhengzhou University,According to the WHO/ISUP classification method,the classification of bladder cancer in 2004,13 cases of low-grade urothelial carcinoma and 14 cases of high-grade urothelial carcinoma,as the control group,16 cases of normal tissues of patients with cancer,and 2cm from the periphery of the cancer,all The source of the specimens has complete medical records,and the specimens have been confirmed by the pathologists of Huaihe Hospital of Henan University,and the two chief physicians have confirmed that the patients have not received any treatments such as chemotherapy or radiotherapy before surgery..Specimens for immunohistochemistry were placed in formalin solution immediately after surgical resection and immediately transferred to a-80 ° C refrigerator.The specimens detected by RT-QPCR were cut into the tissue immediately during the operation,stored in liquid nitrogen,and then transferred to a refrigerator at minus 80 degrees Celsius.Statistical analysis of the experimental data was performed using SPSS 23.0 software.Result1.According to the results of immunohistochemistry,Slit2 was highly expressed in the normal peritumoral bladder cell group,and negatively correlated with tumor malignancy in the tumor group.The positive expression rates of Slit2 in bladder cancer normal tissues,low-grade urothelial carcinoma,and high-grade urothelial carcinoma were 93.8%,53.8%,and 7.1%,respectively(P=0.000),among the three groups.Comparing with each other,there is a significant statistical difference;every two different groups,the degree of expression is compared with each other,still statistically significant.The expression level of Slit2 in normal urothelial carcinoma and urothelial carcinoma of low and high grade groups decreased with the increase of tumor grade(r=0.723,P=0.000).The expression level of Slit2 in the normal urothelial tissue group and the low-grade group decreased with the increase of tumor grade(r=0.464,P=0.026),and Slit2 was in the normal bladder tissue and high-level group.The degree of expression decreased with increasing tumor grade(r=0.866,P=0.000),and the expression level of Slit2 in the low and high levels decreased with increasing tumor grade(r=0.511,P=0.008).2.The levels of Slit2 and internal reference gene transcript m RNA in normal bladder tissue group,low-grade urothelial carcinoma group and high-grade urothelial carcinoma group.The specific amplification curve is shown in the figure below.RT-QPCR detection indicates that Slit2 is normal.The transcriptional m RNA was the highest in bladder tissue,while in urothelial carcinoma,the transcription level decreased with the degree of tumor malignancy,and the highest m RNA was transcribed in high-grade urothelial carcinoma.The three groups were compared(p<0.05).Statistical significance.The comparison between the three groups was still statistically significant.