Expression And Significance Of Slit2/Robo1in Cervical Carcinoma

Objective:To investigate the expression of Slit2and its receptor Robo1in cervical squamous carcinoma (SCC), cervical intraepithelial neoplasia (CIN) and normal cervical epithelium (NCE). Also, explore the role that Slit2/Robo1signal system plays in cervical carcinoma’s generation and development to provide a new outlook for cervical carcinoma’s early diagnosis and treatment.Methods:Immunohistochemistry was used to examine the expression of Slit2and Robo1in55cases of SCC,30cases of CIN Ⅰ-Ⅱ,30cases of CINⅢ20cases of NCE, and the expression of β-catenin and Bcl-2in55cases of SCC. The expression of Slit2mRNA was detected through RT-PCR in20cases of SCC,20cases of CINⅢ,20cases of NCE.Results:1、Slit2positive was defined when brown particles appeared in cytoplasm. Its rate was95%in NCE,but only55%,32.7%in CIN and SCC,respectively.The difference had statistical significance (P<0.05). The positive rate of CIN Ⅲ was much lower than CIN Ⅰ-Ⅱ (P<0.05). The trend of the expression of Slit2mRNA went along with Slit2in all three groups (NCE>CIN Ⅲ>SCC)(P<0.05). Slit2expression in cervical carcinoma was correlated with clinical stage, the depth of invasion, lymphnode metastasis, but not with age, tumor differention and tumor volume.2、Robo1positive was defined when brown particles appeared in cytoplasm. The positive rate was90%in NCE,but only66.7%,40%in CIN and SCC,respectively. The difference had statistical significance (P<0.05).The positive rate of CIN Ⅲ was lower than CIN Ⅰ-Ⅱ(P<0.05).3、The expression of Slit2and Robo1protein in cervical carcinoma was positively correlated (P<0.05)4、The expression of Slit2and β-catenin、Bcl-2in cervical carcinoma was negatively correlated (P<0.05)Conclusion:The normal expression of Slit2and Robo1is essential for the anti-cancer function of Slit2/Robo1signal system. Slit2and (or) Robo1gene silence or expression loss is the early event of the generation of cervical carcinoma and sustains in the development of the carcinoma. Slit2/Robo1signal system’s lossing control of the expression of oncogene is the vital factor of the generation and development of carcinoma.