Preparation And Pharmacodynamics Of Eucommia Male Flowers Granules

Male flower of Eucommia ulmoides was added to the list of ?New Food Raw Materials by the State Health and Planning Commission? in 2014.Studies have shown that E.ulmoides male flowers contain rich active components such as iridoid,phenylalanine,flavonoids and so on,which have many pharmacological effects,such as lowering blood pressure,blood lipid,bacteriostatic,antioxidant,sedation and hypnosis,anti-fatigue,anti-tumor,anti-aging and so on.In addition,E.ulmoides male flowers contain a large number of essential amino acids and minerals,which has certain health care value.E.ulmoides male scented tea was developed and produced from the stamens and flower buds of E.ulmoides.As a kind of health and health tea.Using modern science and technology to extract effective components to make granules is not only convenient to take,nutritious and fully released,and improve the utilization efficiency of male flowers of E.ulmoides Oliv.It is necessary to develop granules of E.ulmoides.In this experiment,the extraction process of E.ulmoides was optimized,the preparation of E.ulmoides was studied,the quality standard was studied and the effect of reducing blood lipid was evaluated in order to improve the benefit of cultivated E.ulmoides and meet the needs of people for health care products.It provides scientific basis for the further development and utilization of male flowers of E.ulmoides.1.Optimization of extraction process of male flower of E.ulmoides.The extraction process of E.ulmoides was optimized on the basis of single factor experiment.The content of chlorogenic acid(70%)and the content of coral glycosides in peach leaves(30%)were used as evaluation indexes.The response surface method was used to optimize the design experiment,and the ratio of material to liquid was investigated.Effects of extraction time,extraction times and ethanol concentration on the extract of E.ulmoides.The optimum extraction process was predicted by Design Exper system as follows: the ratio of material to liquid was 12.27,the extraction time was 60.07 min,the extraction times was 2.13,and the comprehensive score was 89.721.According to the actual situation and maneuverability,the optimum extraction process was adjusted as follows: the ratio of material to liquid was 12 ? 1,the extraction time was 60 min,twice,the extraction temperature was 100 ?,and the concentration of ethanol was 70%.2.Quality Control of male Flower extract of E.ulmoides Oliv.The HPLC method for the quality control of the male flower extract of E.ulmoides Oliv.was used to determine the content of the five active components in the extracts of E.ulmoides,such as the corals of the leaves of the E.ulmoides,and the contents of the five active components of the gynostemma pentaphyllum,the chlorogenic acid,the penheptan acid and the leaves of the vehicle.The results of the validation and the verification of the method are all in accordance with the relevant regulations.According to the results of the three batches,the total cyclic eneether in each gram of E.ulmoides male flower is not less than 4.94 mg,and the chlorogenic acid per gram is not less than 0.35 mg per gram.3.Granulation Technology Male flower of E.ulmoides granules.The granulation process of E.ulmoides male flower granule is used as an inspection index,and the factors such as the air inlet frequency,the air inlet flow,the material temperature,the liquid supply speed,the atomization pressure and the drying time in the one-step granulation process are investigated.The best process for the one-step granulation of E.ulmoides is that the air inlet temperature is 60 ~ 65 ?,the air inlet volume is 50 ~ 55 m h/ h,the atomization pressure is 0.15 MPa,and the rotation speed of the liquid supply is 15r/ min.The obtained granule has the advantages of uniform particle size,good fluidity and good reproducibility.4.Quality Control of Male flower of E.ulmoides granules.The quality control of the male flower granule of E.ulmoides is generally examined according to the relevant regulations of the compendial granules.The results show that the appearance of the male flower of E.ulmoides is dry,the size is uniform,the water,the particle size and the dissolution property meet the requirements of the pharmacopoeia.The effective components of E.ulmoides male flower particles were measured by HPLC,and the methodological validation was carried out.The results of the validation were in accordance with the relevant regulations.According to the results of the 3 batches,the total cyclic enketene in each gram of E.ulmoidesmale flower particles(calculated as the sum of the peach leaf corals,the Beijing-Neillinic acid,the Beijing-Nleveling machine and the vehicle-leaf grass)should not be less than 1.46 mg,and the chlorogenic acid per gram should not be less than 0.10 mg.5.Pharmacodynamic evaluation of the hypolipidemic effect of Male flower of E.ulmoides granules.SD rats were randomly divided into 6 groups: blank group,Lovastatin positive control group,high fat model control group,E.ulmoides granule low dose group(0.26mg/g).Middle dose group(0.50mg/g),high dose group(0.74mg/g).The model was made by giving high fat emulsion,and then the test drug was given.The results showed that compared with the high fat model group,The results showed that E.ulmoides granule had the effect of reducing blood lipid to a certain extent.