Effects Of Sleeve Gastrectomy On Lung Injury In Type 2 Diabetic Rats

ObjectiveDiabetes is one of the diseases that seriously endanger human health,and its incidence rate is increasing.As one of the target organs of diabetes injury,lung damage and its treatment methods have attracted more and more scholars' attention.Studies have shown that treatment with anti-diabetic agents can alleviate lung tissue damage in diabetes.A large number of clinical studies have confirmed that bariatric surgery can improve glucose metabolism quickly and permanently.And the long-term therapy effect of bariatric surgery on diabetes is significantly better than traditional medical treatment.As a widely-developed bariatric surgery,sleeve gastrectomy has the characteristics of simple operation and less postoperative complications,accounting for 45.9%of the total weight loss surgery in the world,and the amount of this surgery is increasing year by year.So far,there has been no research around the world to investigate whether sleeve gastrectomy can alleviate lung tissue damage caused by diabetes.In this study,the type 2 diabetic rats underwent sleeve gastrectomy.Then we observed the morphological findings and detected related indexes of lung tissue in type 2 diabetic rats after sleeve gastrectomy in order to examine the sleeve gastrectomy's remission.effect of lung injury in type 2 diabetic rats and elucidate its underlying mechanisms.MethodsThe Wistar rats were induced diabetes through high fat diet(HFD)feeding combined with injecting streptozotocin(STZ)intraperitoneally.Diabetic rats were randomly assigned to the sleeve gastrectomy group(SG group)and sham group.Body weight,food intake,fasting blood glucose and glucose tolerance were measured postoperatively.HE staining was performed to observe the histological appearance of lung.The expression of inflammation factors Interleukin-6(IL-6)?Tumor Necrosis Factor ?(TNF-?)and oxidative stress indicator Nitrotyrosine(NT)were determined by Western blotting and immunohistochemistry.The expression and activity of oxidative stress indicators Nitric Oxide(NO)?Malondialdehyde(MDA)?Superoxide Dismutase(SOD)were determined by biochemical testing.ResultsOnly one rat of SG group died after surgery.SG group exhibited improvement in glucose control compared with sham group postoperatively(P<0.01).The thickness in interalveolar septum and the hemorrhage in alveolus in SG group were relieved compared with sham group.The expressions of IL-6?TNF-a?NT?NO and MDA were decreased in SG group(P<0.01).The activity of SOD was increased in the SG group at the same time(P<0.01).ConclusionSleeve gastrectomy has remission effect on the lung injury of diabetic rats.Its effect may be due to the decreased expression of inflammation factors and amelioration of oxidative stress status in lung via ideal glycemic control.