Analysis Of Characteristics Of Intestinal Florain Patients With First-episode Depression

BackgroundThe pathogenesis of depression is complex,and it is generally believed that genes and environment and their interactions lead to clinical morbidity.Intestinal flora is not only regulated by host genes,but also changes with diet and environment.At the same time,the microbiome-gut-brain axis theory is increasingly accepted.Therefore,the role of intestinal flora in the pathogenesis of depression has received increasing attention.There are more and more studies on the characteristics of intestinal flora in patients with depression,however,there are few studies on the characteristics of intestinal microflora in patients withfirst-episode depression.This topic is to explore the characteristics of intestinal flora in patients with first-episode depression.PurposeTo explore the characteristics of intestinal flora of patients withfirst-episode depression,so as to enrich our understanding of the intestinal flora of depression and provide reference for clinical application.MethodBy adopting the method of case-control study,in accord with the DSM-? diagnostic criteria of 27 patients with first-episode depression(group A),men in 11 cases,16 cases of women.30 normal controls(group B)with matching gender,age,education level and body mass index were included,including 13 males and 17 females.All the above objects are screened in strict accordance with the inclusion criteria and exclusion criteria.For the enrolled subjects,their age,gender,bmi,irritable bowel syndrome(IBS),smoking and alcohol consumption were recorded.Fresh stool samples were collected from all subjects within 24 hours after the end of the HAMD scale assessment.Deoxyribonucleic acid(deoxyribonucleic acid)was extracted from the stool samples of subjects,and specific primers were used to amplify the v3-v4 region of bacterial 16 S rRNA.Sequencing through high-throughput sequencing(HTS).Trimmomatic,QIIME,Flash,Mothur,Vsearch,USEARCH and other software were used to analyze the sequencing results.SPSS23.0software was used for statistical analysis of the experimental data.ResultsFinally,26 patients with first-episode depression and 26 normal controls were included in the analysis.The incidence of IBS in patients with first-episode depression is more prominent(IBS is excluded before the first depression).There was no significant difference in the diversity index between the first depression group and the control group(P>0.05).The abundances of Chloroflexi,Cytophagia,Enterococcaceae,and Bacillus_coagulans were higher in the first depression group than in the control group.Group(P<0.05).Using the LDA bar chart,we found that Bacteroides,Weissella,Leuconostocaceae,and Plasmodium were significantly reduced in the intestines of patients with first-episode depression.ConclusionOn the whole,there was no significant difference in the diversity and abundance of intestinal flora between patients with first-episode depression and normal people.Importantly,in terms of some strains,our study revealed significant differences between the two groups of subjects.The first depressive patients had both a reduction in beneficial bacteria and an increase in harmful bacteria and very few beneficial bacteria,while prevotella can be regarded as a possible biomarker for monitoring depression.This has enriched our understanding of the intestinal flora of depression,and also indicated that changes in intestinal flora may affect the incidence of depression and play a role in this process.