The Study On The Relationship Between Intestinal Microbiota And Schizophrenia

The background and significance:The global high prevalence of schizophrenia,brings heavy burden to the society,takes up a lot of medical resources,has become an important global public health problem.At present the treatment of schizophrenia are mainly symptomatic treatment,have some limitations.A growing body of evidence indicates that gut microbiota were associated with schizophrenia occurrence,development,through pathway consist neurotransmitters,immune factors,metabolic risk factors.Therefore,intestinal flora and schizophrenia,the relationship between the occurrence and development of schizophrenia for opening up the new way of mental crack disease treatment.Objective:1.Analysis of patients with schizophrenia and normal human intestinal microbiota,to investigate the changes of intestinal microbiota at phylum,species and genus level in patients with schizophrenia.2.Analysis of the changes of patients with schizophrenia with metabolic markers,in order to investigate the correlation between metabolic markers and schizophrenia severity.3.Correlation analysis between patients with schizophrenia and changes of intestinal microbiota metabolism,so as to explore the effect of intestinal microbiota in the occurrence and development of schizophrenia.Methods:1.According to the clinical diagnostic criteria,patients with schizophrenia were selected.2.Fecal and blood samples of normal people and psychiatric patients were collected.3.The differences of intestinal microbiota in normal people and psychiatric patients were distinguished by next geneproportionn sequencing technology.4.Using bioinformatics methods to explore the important microbiota which is closely related with the occurrence and progression of schizophrenia.5.Measured blood lipids,liver and renal function,blood glucose and other indicators in normal people and patients with schizophrenia.6.Analyzed the relevance of metabolic indexes and schizophrenia SCL-90 score by SPSS.7.Using SPSS,the correlation between intestinal microbiota of patients with schizophrenia and metabolic markers was also discussed.Results:1.In view of the number of opeproportionnal taxonomic units(OTU),the abundance of bacteria,and the bacterial diversity analysis,the intestinal microbiota diversity in patients with schizophrenia had no significant difference comparing to the normal people.2.Different than that of normal population,the proportion of bacteroidetes and firmicutes(B/F)in patients with schizophrenia had the tendency to rise.On the contrary,the proportion of Proteobacteria increased obviously(p<0.05),the proportion of Actinomycetes decreased obviously(p<0.01)and the proportion of Fusobacteria increased obviously(p<0.05).3.Compared with normal people,the differences in genus level was mainly presented that Faecalibacterium decreased remarkably(p<0.001),Pseudobutyrivibrio decreased significantly(p<0.05)and Lactobacillus increased notably(p<0.01).Bifidobacterium in Actinobacteria decreased notably(p<0.01),Escherichia-Shigella bacteria in Proteobacteria increased significantly(p<0.001).4.Comparing to normal people,the level of blood triglyceride(TG),ALT and creatinine in the schizophrenic patients were significantly higher(p<0.01);liver TBIL level decreased significantly(p<0.01);renal urea nitrogen levels increased significantly(p<0.05).5.The content of TG and schizophrenia SCL-90 score showed a moderately positive correlation(r=0.46).6.Proteobacteria and TG was showed a low degree of positive correlation(r=0.35),Escherichia-Shigella bacteria and TG was presented a moderately positive correlation(r=0.52),Lactobacillus and TG was indicated a low degree of positive correlation(r=0.35).Conclusion:1.Compared with normal people,patients with schizophrenia had no change of intestinal microbiota abundance and diversity,but the structure of intestinal microbiota had changed at the phylum and genus level.2.The content of blood TG in patients with schizophrenia had a moderately positive correlation with the severity of schizophrenia.3.The change of specific intestinal microbiota had a moderately positive correlation with the content of blood TG,which confirmed that the intestinal microbiota had an effect in the occurrence and development of schizophrenia.