Clinical Analysis Of T Lymphocyte Subgroup Count In Patients With Cervical Cancer Treated With Radiotherapy

Objectives:To investigate correlation between T-lymphocyte subsets in peripheral blood and the major clinic-pathological characteristics in cancer patients.Methods:Selection of 60 cases of hospitalized patients in September 2016-April2018 in professor Xu'treatment group in tumor radiotherapy department of The Second Hospital of Dalian Medical University,all patients were early treatment of female patients with tumor radiotherapy or not has lines of radiation and chemotherapy but can be in The Second Hospital of Dalian Medical University in the system to find out the lymphocyte subsets of radiotherapy before the results of the female patients with tumor,the pathologic results and clinical data integrity.The age of onset,tumor volume,clinical stage,tissue grade,pathological type and radiotherapy were collected.Data of 60 women in the normal control group were randomly selected from the healthy population in physical examination center of The Second Hospital of Dalian Medical University,without previous medical history of immune system and blood system.Immunofluorescence staining and Flow cytometry(FCM)were performed to detect the T lymphocyte subsets in the peripheral blood of patients and healthy women.Peripheral venous blood was collected and detected in 43 cases of squamous cell carcinoma,10 cases of adenocarcinoma,6 cases of adenosquamous carcinoma and1 case of malignant melanoma before the initial treatment and at the end of radiotherapy,respectively.Sixty patients,aged from 31 to 85 years old,were analyzed.The t-lymphocyte subsets of cervical cancer patients and normal subjects were analyzed using SPSS 19.0 software.The absolute count of T lymphocyte subsets before and after radiotherapy was statistically analyzed.The correlation between the main clinical features and the t-lymphocyte subsets of cervical cancer patients before initial treatment.Results:1.CD8~+in T lymphocytes of patients with cervical cancer before initial treatment was higher than that of healthy controls(P<0.01).The absolute count of CD3~+?CD4~+and CD4~+/CD8~+was significantly lower than that of the healthy control group(P<0.01).2.There was no significant correlation between the changes of T lymphocytes and age in patients with cervical cancer.3.The absolute count of CD3~+?CD4~+?CD8~+were significantly lower in patients with stage ?-? cervical cancer(p<0.01),but there was no significant difference between the two groups(p>0.05).4.There was no clear correlation between pathological type and T cell subgroup,but CD8~+was significantly lower in adenosquamous cell carcinoma patients than in adenocarcinoma patients(p<0.05).CD4~+/CD8~+was significantly higher in squamous cell carcinoma patients than in adenocarcinoma patients(p<0.05).5.CD3~+,CD4~+,andCD8~+counts in patients with low-moderately differentiated cervical cancer were significantly lower than those in the group with highly differentiated tumor(p<0.01),but there was no statistically significant difference in CD4~+/CD8~+(p>0.05).6.Absolutely the count of CD3~+?CD4~+?CD8~+and CD4~+/CD8~+in patients with cervical cancer after radiotherapy were significantly lower than those before initial treatment(p<0.01).Conclusions:The T cell-mediated immune function in patients with cervical cancer was inhibited before starting radiotherapy,and was closely related to the clinic-pathological features related to early diagnosis and prognosis,providing evidence that an early supporting therapy to improve the patient's immune function would be necessary.