The Association Between The CaSR Gene Polymorphism And Idiopathic Calcium Oxalate Stones Of Uighur And Han Population In Xinjiang Region

Objective:To investigate the correlation of calcium sensitive receptor(CaSR)gene polymorphism with the incidence of idiopathic calcium oxalate stones in Uyghur and Han population in Xinjiang.Methods:398 cases of ICOS patients(200 case of Uyghur and 198 case of Han)and 399 cases of controls(200 controls of Uy-ghur and 199 controls of Han)were selected in this study.The CaSR gene rs104-2636,rs1801726 polymorphism was analyzed by the methods of SnaPshot and the genotype distribution was compared between different groups.The serum calcium and 24h urinary calcium excretion levels were compared among different genotypes in both case and control groups.Results:Single Nueleotide Polymorphism(SNP)rs1042636,SNP rs 1801726 of CaSR gene were detected,in case group and control group of each nationalities,the genotype frequencies of the two locis are in compliance with Hardy-Weinberg equilibrium.There was no significant difference found in genotype frequencies and allele of rs1042636 and rs1801726 locis between case and controls in both Hans and Uyghurs(P>0.05).For CaSR gene rs1042636 polymorphism respectively in the cases and controls,the statistical result of its genotype was(cases:P=0.001;controls:P=0.024),the A allele in Uyghur both in cases and controls was higher comparing with those Hans,the Statistical results of its allele was in cases:OR=2.145 95%CI=[1.602-2.866]P<0.01,in controls:OR =1.77-3 95%CI=[1.332～2.359]P<0.01,allele frequencies of Uyghur/Han in case group were A=278(69.5%)/204(51.5%),G=122(30.5%)/192(48.5%);Uyghur/Han in control group were A=264(66.0%)/208(52.3%),G=136(34.0%)/190(47.7%).However,for CaSR gene rs1801726 the genotype frequencies and allele was no significant difference between two ethnic groups.Furthermore,we also found that in Hans population the individuals who carried genotype AG+GG had the higher urinary calcium than who carriers AA genotype,(in Han case:P=0.007 and in Han total:P=0.01),in Uyghur population the serum calcium and 24 hour urinary calcium had no significant difference among different genotype of CaSR gene two SNP.Conclusion:The rs042636,rs1801726 polymorphisms of CaSR Gene had no associated with the risk of idiopathic calcium oxalate stones in Xinjisng region.In Han population,CaSR gene rs1042636 polymorphism had effect on the urinary calcium excretion,it may be one of the genetic factors for regulating the calcium excretion.