Effect Of Boschniakia Rossica Polysaccharides On LPS-inducded Inflammation Of RAW264.7 Macrophages

Objective:To investigate the effect and mechanism of Boschniakia rossica polysaccharides(BRPS)on inflammatory response of RAW264.7 macrophages induced by lipopolysaccharide(LPS).Methods:RAW264.7 macrophages and L02 hepatocytes were stimulated by LPS to establish an in vitro macrophage and hepatocyte inflammation model.Toxicity of BRPS to RAW264.7 macrophages and L02 hepatocytes was detected by cell proliferation toxicity assay,the secretion of interleukin-1?(IL-1?)and interleukin-6(IL-6)and tumor necrosis factor-a(TNF-a)of on LPS-induced RAW264.7 and L02 cells was detected by enzyme-linked imnuinosorbent assay(ELISA).Western blot was used to detecte the expression and/or activation of cyclooxygenase-2(COX-2),STAT3,mitogen-activated protein kinase(MAPK),and nuclear transcription factor-KB(nuclear factor-KB,NF-?B)pathway proteins in LPS-induced RAW264.7 cells.Result:After treatment of LPS of different concentrations,the survival rates of RAW264.7 macrophages and L02 hepatocytes were not significantly different from that of the normal group(P>0.05).However,LPS at the concentrations of 100?2000 ng/mL could significantly up-regulate the expression of COX-2 protein in RAW264.7 cells(P<0.01),suggesting that the macrophage inflammation model was established.LPS could up-regulate the expression of COX-2 protein in L02 cells(P<0.01),but to a lesser extent than that of RAW264.7 macrophages.2.BRPS had no significant effect on the survival rate of RAW264.7 cells and L02 cells at the concentrations of 0?100 mg/L(P>0.05),suggesting its non-cytotoxic characteristic.3.Compared with the normal group,the secretion of IL-1?,IL-6 and TNF-a were significantly increased in the RAW264.7 inflammatory model(P<0.01).Compared with the model group,the secretion of IL-1?,IL-6,TNF-a was significantly decreased(P<0.05)in the BRPS group in a dose-dependent manner.There was no significant change in the secretion of IL-1?,IL-o and TNF-a in L02 inflammatory model(P>0.05).4.Compared with the normal group,the expression of COX-2 protein in RAW264.7 inflaramatory model was significantly increased(P<0.01).Compared with the model group,the expression of COX-2 protein in BRPS group was significantly decreased(P<0.05).5.Compared with the normal group,the expression of MyD88,IRAK1,p-NF-?B,NF-?B,p-ERK,p-JNK,p-p38,and p-STAT3 were significantly increased in the RAW264.7 inflammatory model(P<0.01).Compared with the model group,the levels of MyD88,IRAK1,p-NF-?B,NF-?B,p-ERK,p-JNK,p-p38,and p-STAT3 in the BRPS group were significantly reduced(P<0.05).Conclusion:BRPS can inhibit the inflammatory response of RAW264.7 macrophages induced by LPS,and its mechanism may be related to the inhibition of NF-?B and MAPK signaling pathways.