Effect Of Iridoid Glucosides From Boschniakia Rossica On Liver Marker Enzymes And Antioxidative Enzymes Of Rats With Hepatic Preneoplasia

Objective:To study the effect of iridoid glucosides from Boschniakia rossica (IGBR) on liver tissue homogenate,Mitochondrial Marker Enzyme and antioxidative enzymes of rats with hepatic preneoplasia induced by diethylnitrosamine (DEN) and investigatc the possible mechanism of its anti-tumor.Methods:Sixty male Wistar rats were randomly assigned into five groups, each groupHave 12 rats. control group [saline-basal diet (BD)-partial hepatectecomy (PH)], model group[(DEN-acetylaminoflofluorene(AAF)-PH)], IGBR low dose group (DEN-AAF+125mg/kgIGBR-PH), IGBR medium dose group (DEN-AAF+250mg/kg IGBR-PH) and IGBR high dose group (DEN-AAF+500mg/kg IGBR-PH). Observe the change of hepatic in rats, and make liver tissue homogenate and Hepatic mitochondria after the eight week execute rats, determination of the content of aspartate amino-transferdse (AST), alanine trasaminase (ALT), y-glutamy transpeptidase (y-GT), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), glutathione-S-transferase (GST), and malondialdehyde (MDA).Result:IGBR obviously improved the weigh gain, mental state, color pattern for rats. the rats in model group liver tissue homogenate AST,ALT,γ-GT,GST activity and MDA level were increased,and the SOD,CAT, GSH-PX activities were dereased in model group before hepatic preneoplastic; compare with model group, the mitochondria in liver GST activity and MDA content were increased and SOD,CAT,GSH-PX activity were decreased. IGBR liver tissue homogenate reduced the activities of y-GT,ALT,AST,GST and MDA content, SOD,CAT,GSH-PX activity firm up; GST activity and MDA content were reduced, and SOD,CAT,GSH-PX activity have firm up in liver tissue homogenate.Conclusion:IGBR exhibited obvious protection effect on DEN induced rats hepatic preneoplastic lesions, prevent its lipid peroxidation and enhance antioxidant enzymes activity.