The Effect And Clinical Observation Of Drynariae Rhizome On Osteogenic Differe Ntiation Of BMSCs Of Ovariectomized Rats Based On "Marrow Atrophy Bone Withered" Theory

Objective: Under the guidance of the theory of“Marrow Atrophy Bone Withered”,the clinical characteristics of postmenopausal osteoporosis(PMOP)in postmenopausal osteoporosis are discussed,and the basic pathogenesis and treatment of PMOP are discussed in combination with the literature of previous dynasties,and the re bone in the prevention and treatment of the osteoporotic compression fracture of the thoracic and lumbar vertebrae after menopause in female postmenopausal women is discussed.The effect of fracture was observed,and the effect of osteoblast on osteogenic differentiation of bone marrow mesenchymal stem cells(Bone Mesenchymal Stem Cells,BMSCs)in ovariectomized rats was investigated based on the theory of pulp dry bone flaccid and Wnt/beta-Catenin signaling pathway,and the prevention and treatment of PMOP from the level of cell molecules was studied from the level of cell molecules.The mechanism will provide theoretical and experimental evidence for better application of Rhizoma Rhizoma of Rhizoma Rhizoma.Methods:Divided into three parts:First,experimental research1.Female Sprague-Dawley(SD)rats were used as experimental subjects,and ovariectomized rats were used to establish osteoporosis model.Eight ovariectomized groups,eight ovariectomized groups and eight Sham operation group three as a control.After 3 months of modeling,double-energy X-ray absorptiometry(small animal model)was performed to observe the bone mass.2.Make dry decoction,weigh 250 g dried decoction,add three distilled water to the height of Chinese medicinal herb,soak at room temperature for 30 min,change the simmer for 20 min after boiling,then keep the liquid;Add water to the height of the Chinese medicine Pieces,the two fried after the fire Wenjian boiled boiled 30 min,keep the liquid,two decoctions combined,double gauze filtration,concentrated to 250 ml,aliquoted and-20 ? refrigerator for later use.3.Each rat by 2ml / day gavage for 3 months.In vitro cultured BMSCs.Flow cytometry identification.ALP activity,alizarin red staining.Westen-Blot detection of Wnt / ?-catenin signaling pathway related protein expression.8.RT-PCR detection of related osteogenic gene expression.Second,Chinese literature research From ancient medical writings to sort out the understanding of PMOP,in order to sort out myeloid atrophy theory in the literature,analysis of PMOP TCM etiology and pathogenesis,summarizes the history of physicians PMOP treatment,the study of Bu Ke Bu Bu PMO treatment feasibility.Third,clinical research(1)138 female patients with thoracolumbar osteoporotic compression fractures hospitalized in the Department of Spine Orthopedics,Affiliated Hospital of Shandong University of Traditional Chinese Medicine were enrolled in this study.Basic clinical information was collected before operation.The patients were treated with dual-energy X-ray absorptiometry(Bone Mineral Density,BMD)(?-2.5cm2 / g)were measured in the right forearm,left hip and lumbar spine.The clinical symptoms were observed and the TCM syndromes were summarized.(B)of the selected patients were randomly divided into observation group and control group,the observation group was given oral Qiangu capsule,salmon calcitonin,calcium carbonate D3 tablets,the control group given oral salmon calcitonin,calcium carbonate D3 tablets.The two groups were treated for 6months.To observe the clinical treatment,whether to fracture again,the time of further fracture,the number of falls,ODI score and VAS score.Conclusion:1.The osteogenic potential of BMSCs in ovariectomized group was higher than that in ovariectomized group,Wnt / ?-catenin signaling pathway was relatively open compared with that of ovariectomized group,and there was more bone gene expression in ovariectomized group than in blank group,Which played a role in anti-osteoporosis.2.In the prevention of thoracolumbar osteoporotic compression fractures and fractures,Qiangu capsule combined with calcium carbonate D3 tablets can significantly improve the quality of life of patients to relieve pain and reduce the number of falls,and relatively effective in avoiding fractures,reached the PMOP treatment.3.Osteoporosis can not only enhance the osteogenic capacity of BMSCs in ovariectomized rats,but also improve the bone strength of the patients and increase the weight-bearing capacity of the bone,-Strong tendons purpose.