| OBJECTIVES:By using the application ofsmall samples for high throughput gene chip and large samples qRT-PCR for validation,to find the 1ong non-coding RNA in gastric cancer.To find the relationship between long chain noncoding RNA and pathological factors of gastric cancer.To study the effect of lncRNA on the biological function of gastric cancer cells.METHODS:1)Fresh samples of paired gastric cancer and adjacent normal tissues from 6 gastric cancer patients,were used for LncRNA gene chip.2)The lncRNA gene chip results were verified by qRT-PCR in fresh frozen samples of paired gastric cancer and adjacent normal tissues from 89 gastric cancer patients.3)The relative expression of target lncRNA in 89 gastric cancer and adjacent tissues was detected by qRT-PCR.Combined with the clinical pathological factors,the correlationship between target LncRNA and Clinicopathological features in gastric cancer patients was compared and studied.4)After infection of plasmid-lncRNA RP11-335I12.2 in SGC-790 1 and BGC-823 gastric cells,the stable overexpression gastric cancer cells were validated by qRTPCR.The changes of gastric cancer cell functions were assessed by experiments of cell proliferation,migration in vitro.RESULT:1)Gene chip and qRT-PCR results showed that the expression of lncRNA RP11-335I12.2 were significantly lower in the gastric cancer tissues relative to paired adjacent tissues.2)The relative expression level of lncRNA RP11-335I12.2 in gastric cancer tissues was closely correlated with tumor size,depth of cancer in vasion,lymphatic metastasis and perineural invasion.3)LncRNA RP11-335I12.2-plasmid was successfully stably transfected into SGC-7901 and BCG-823 cell lines which Showed the lowest expression levels of lncRNA RP11-335I12.2 among 5 gastric cancer cell lines?CCK-8 experiments showed the proliferation of SGC-7901 and BCG-823 cells was significantly inhibited in lncRNA RP11-335I12.2-plasmid groups as compared with plasmid-NC group.The results of tablet colony forming experiment showed that the colony forming efficiency was significantly decreased in lncRNA RP11-335I12.2-plasmid SGC-7901 cells and in lncRNA RP11-335I12.2-plasmid BCG-823 cells as compared with corresponding plasmid-NC cells?Transwell migration assay indicated the penetrated cell number of lncRNA RP11-335I12.2-plasmid BCG-823 cells and SGC-7901 was significantly less than plasmid-NC-BCG-823 cells and SGC-7901.CONCLUSION:1)The expression level of lncRNA RP11-335I12.2 in tumor tissues of gastric cancer patients was significantly lower than that in adjacent normal tissues.2)The expression of lncRNA RP11-335I12.2 in gastric cancer tissues was closely related with gastric cancer tumor size,depth of cancer invasion,lymphatic metastasis and perineural invasion.3)LncRNA RP11-335I12.2 could inhibit proliferation and migration of gastric cancer cells. |
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