Functional Study Of Long Chain Non-coding RNA RP11-452H21.4 In Pancreatic Cancer Cells

Objective: To investigate the effects of silent and overexpressed long-chain noncoding RNA RP11-452H21.4 on the malignant phenotype of pancreatic cancer cells,and to lay a foundation for elucidating the mechanism of pancreatic cancer.Methods: Smart Silencer and eukaryotic expression vector pcDNA3.1targeting long-chain non-coding RNA RP11-452H21.4 were constructed and transfected into pancreatic cancer cell line Panc-1.The knockdown and overexpression effects were identified by reverse transcription polymerase chain reaction(RT-PCR).The following three cell experiments were carried out on pancreatic cancer cell line Panc-1 in vitro:(1)CCK-8 method was used to detect the change of proliferation of pancreatic cancer cell line Panc-1;(2)The migration ability of pancreatic cancer cell line Panc-1 was detected by transwell migration experiment;(3)Transwell invasion assay was used to detect the invasion ability of pancreatic cancer cell line Panc-1.Results:(1)Reverse transcription polymerase chain reaction(RT-PCR)showed that the knockdown and overexpression of long-chain non-coding RNA RP11-452H21.4 in Panc-1 pancreatic cancer cell line weresignificant.(2)CCK-8 proliferation experiment showed that the proliferation rate of pancreatic cancer cell line Panc-1 in the knockdown group of long-chain non-coding RNA RP11-452H21.4 was not significantly different from that in the control group at 24 h and 48 h,but in the knockdown group of long-chain non-coding RNA RP11-452H21.4at 72 h and 96 h,the proliferation rate of pancreatic cancer cell line Panc-1was significantly higher than that in the control group,and the difference was statistically significant(2.16�0.20 Vs 1.59�0.15,P=0.017;2.62�0.20 Vs 1.99�0.12,P=0.021);The proliferation rate of Panc-1 cell line in the high expression group of long-chain non-coding RNA RP11-452H21.4 and empty plasmid group was not significantly different at 24 h and 48 h,while the proliferation rate of Panc-1 cell line in the high expression group of long-chain non-coding RNA RP11-452H21.4 at 72 h and 96 h was significantly lower than that in the high expression group of empty plasmid group(1.23�0.07 Vs 1.57�0.11,P=0.009;1.46�0.17 Vs 1.89�0.17,P<=0.035).(3)Transwell migration experiment showed that the number of pancreatic cancer cell line Panc-1 perforating cells in the long-chain non-coding RNA RP11-452H21.4 knockdown group was significantly higher than that in the control group(180.33 � 17.17 Vs79.67�8.80,P=0.002);the number of pancreatic cancer cell line Panc-1perforating cells in the long-chain non-coding RNA RP11-452H21.4 high expression group was significantly lower than that in the empty plasmidgroup,and the difference was significant(61.33�10.96 Vs 91.33�10.50,P=0.049).(4)Transwell invasion experiment showed that the number of PANC-1 perforating cells in long-chain non-coding RNARP11-452H21.4knockdown group was significantly higher than that in control group(130.33 � 13.07 Vs 85.67 � 10.96,P=0.021);the number of PANC-1perforating cells in long-chain non-coding RNARP11-452H21.4 high expression group was significantly lower than that in empty plasmid group,and the difference was significant(35.67�8.96 Vs 83.00�10.20,P=0.008).Conclusions:(1)By down-regulating the expression of long-chain non-coding RNA RP11-452H21.4 in pancreatic cancer cell line Panc-1,the cell proliferation,migration and invasion increased;Upregulation of the expression of long-chain non-coding RNA RP11-452H21.4 in Panc-1cell line resulted in a decrease in cell proliferation,migration and invasion.(2)Long-chain non-coding RNA RP11-452H21.4 can inhibit the proliferation,migration and invasion of pancreatic cancer cell line Panc-1.