| Part One The expression of mi RNA-361 in the Tissue of Oral Squamous Cell Carcinoma and the relationship between the expressions and the clinicopathological characteristics of patientsObjective:To examine the expression of miRNA-361 in Oral cancer tissue and normal oral mucosal tissue by Taqman real time PCR and study the relationship between the expressions and the clinicopathological characteristics of patients.Methods:1 The expression level of miRNA-361 in OSCC and normal oral mucosa were detected by RT-Qpcr.The correlation between the expression level and age,sex,clinical stage and cervical lymph node metastasis were studied2 SPSS21.0 software was used to analysis experimental data.The t test was used to compare the differences between the experime-ntal data of each group,and P<0.05 was considered to have the dif-ference of statistics,.Results:1.The expressions of miR-361 in OSCC tissue and matched normal mucosa tissue were 10.93±3.32,13.68±2.47 respectively.there were significant differences(P <0.05).2.The expression of miR-361 was correlated with pathological type.The expression of miRNA-361 in Ⅰ,Ⅱ and Ⅲ,Ⅳ were 12.31±3.14,9.00±2.57,P=0.002,and with lymphatic metastasis and without lymphatic metastasis were 9.66±2.88,12.21±3.30,p=0.019.But its expression was not correlated with ages,sex.Brief Summary:The expression level of miR-361 in normal tissue were higher than in OSCC,Which suggest that miR-361 may be related to degree of malignancy as a oncogene.The expression level of miR-361 was significantly correlated with histologic grade,lymphatic metastatic,suggesting that miR-361 might play an important role in the occurrence and development of OSCC,which may be a promising biomarker in the early diagnosis of OSCC.Part Two Study of the effect of miRNA-361 on the biological function of TCA-38 cellsObjective:To investigate the influences of miRNA-361 on proliferation and migration of TCA-38 and to explore the role of mi RNA-361 in carcinogenesis and lymphatic metastasis of OSCC.To evaluate diagnosis value of miRNA-361 for OSCC as tumour marker,elucidate the biological function of miR-361 in the development of tumor,provide theoretical and experimental basis for early diagnosis of OSCC.Methods:1 The mi R-361 mimic/negative miR-361 were transfected into OSCC lines TCA-83 for 24 hours.At the same time blank control group was set.The expression of mi RNA-361 in three groups of cells were detected by RT-qPCR.2 MTS colorimetric analysis was used to detect the OD of 1d,2d,3d,4d,and 5d,and the cell growth curve was plotted.3 We transfected the miR-361 into OSCC cell line,and observe-d the cloning ability of OSCC cells..4 Transwell assay was used to detect the effect of miRNA-361 on migration of OSCC cell line TCA-83.5 The data were analysised by spss21.0 software and were exp-ressed as mean ± standard deviation.The t-test was used for paire-d samples and Mann-Whitney test was used for independent sample-s between the two groups.Results:1.The expression of miR-361 in experimental group higher thanthat in control group and blank group after 24 h of transfection.2.After 1,2,3,4,5 day of transfection,the OD value by MTS assay did not show statistical significance between blank group and control group in TCA-83 cell line;but the OD value in experimental group were higher than that in control group at every interval of 24 hours,there were significantly differences.3.Cell number breaking through the transwell of TCA-38 transfected mi R-361 mimics is significantly lower than the negative group and blank group,and the difference was statistically significance(P<0.05).4.Cell clonality of TCA-38 transfected miR-361 mimics is significantly lower than the negative group and blank group,and the difference was statistically significance(P<0.05).Brief Summary:miR-361 expression level is descend in cell of TCA-38,and it is closely associated with the transfer and prognosis of the cancer.The chang of miR-361 can significantly affect the biological behaviorof OSCC,such as: proliferation,invasion,metastasis.Conclusions:miR-361 expression level is reducted in OSCC tissue,and it is closely associated with the transfer and prognosis of the cancer.The chang of miR-361 can significantly affect the biological behavior o-f OSCC cell,such as: proliferation,invasion,metastasis. |
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