The Effects Of K_Ca Channels On Biological Behavior Of Trophoblasts

?Objective?The aim of the study was to determine K_Caa channels expression in human villous trophoblasts and their role in proliferation,apoptosis and invasiveness of HTR-8/SVneo cells and human villous trophoblasts at fist term pregnancy;and ultimately to improve the pathogenesis of preeclampsia.?Methods?1 BK_Ca channels?subunit and?1 subunit,IK_Caa channels,SK_Caa channels expression in villous trophoblasts at fist term pregnancy was described using immunochemistry analyses.2 BK_Ca channels?subunit and?1 subunit,IK_Caa channels,SK_Caa channels expression in HTR-8/SVneo cells was described using cell culture immunofluorescence analyses.3 HTR-8/SVneo cells viability was measured by cell counting kit?cck-8?assay in the presence of NS1619(BK_Ca channels activitor),ibTX(BK_Ca channels blocker),NS309(IK_Caa channels and SK_Caa channels activitor),Apamin(SK_Caa channels blocker)and TRAM34(IK_Caa channels blocker).4 Apoptotic rate of HTR-8/SVneo cells was measured by flow cytometry analyses in the presence of NS1619,ibTX,NS309,Apamin and TRAM34.5 Invasive ability of HTR-8/SVneo cells was measured by transwell method in the presence of NS1619,ibTX,NS309,Apamin and TRAM34.?Results?1 BK_Ca channels?subunit and?1 subunit,IK_Caa channels,SK_Caa channels were expressed at cytomembrane and cytoplasm in villous trophoblasts at fist term pregnancy.2 BK_Ca channels?subunit and?1 subunit,IK_Caa channels,SK_Caa channels were expressed at cytomembrane and cytoplasm in HTR-8/SVneo cells.3 Activating BK_Ca channels,IK_Caa channels,and SK_Caa channels promoted HTR-8/SVneo cells proliferation;while blocking their function not brought obvious effection.4 Enhanced activities of BK_Ca channels was found to be correlated with increased apoptosis of HTR-8/SVneo cells,whereas enhanced of IK_Caa channels,SK_Caa channels and inhibition of BK_Ca channels,IK_Caa channels,and SK_Caa channels were not significantly affect apoptosis of HTR-8/SVneo cells.5 Enhanced activities of BK_Ca channels,IK_Caa channels,and SK_Caa channels were found to be correlated with inhibition invasivenessof HTR-8/SVneo cells,whereas blocking of BK_Ca channels,IK_Caa channels,and SK_Caa channels were not significantly affect invasiveness of HTR-8/SVneo cells.?Conclusion?The pressent results suggest that K_Ca channels are expressed in villous trophoblasts at fist term pregnancy.K_Ca channels play an important role in proliferation,apoptosis and invasiveness in HTR-8/SVneo cells.Taken together our results indicate that K_Ca channels may be considered an important target related to the action of villous trophoblasts at fist term pregnancy in spiral arteryremolding and may play a role in pathogenesis of preeclampsia.