| Acer mono is widely distributed in the northeast and north of China,it also distributed in other countries.There are many variations of A.mono.The researches on A.mono are focus on the development and utilization of lumber,landscaping and breeding technique.Leaves of A.mono contain stilbene glucoside which can protect liver.The efrect of stilbene glucoside on protecting liver is higher than that of silymarin.A.mono is called Wulongpi in Chinese Herbal Medicine Assembly.its branches and leaves are used for dispelling wind and eliminating danpness,and promoting blood circulation to arrest pain.Samara of A.mono is riched in oil which contains nervonic acid.Nervonic acid has recovery effect on the nerve fibers.Samara oil is often used for industrial raw material and edible oil.But,there are few research reports on the use of sustainable parts of A.mono.In this paper,the seeds of A.mono was used as raw material to develope a method for extracting samara oil,gallic acid and resveratrol of A.mono.In order to increase the extraction yield of gallic acid and resveratrol,macroporous resins were used for purification and separation of gallic acid and resveratrol.The main content 1s as follows:(1)The supercritical CO2 method was used for extracting the A.mono samara oil.The supercritical time,pressure,CO2 flow rate and temperature were investigated by single factor method and Box-Behnken response surface optimization method.The optimal conditions for extracting samara oil were as follows:the extraction time is 147 min,the extraction pressure is 36 MPa,the CO2 flow rate is 98 mL/min,the extraction temperature is 47?.The average yield of A.mono samara oil is 71.3 mg/g.The obtained samara o1l was analyzed by GC-MS and NIST standard spectral library to compare the corresponding mass spectrum of various peaks.The retention time is 57.15 min,the peak area is 464057,the proportion is 1.03%.(2)It has two matters of gallic acid and resveratrol which isolation and identification from resveratrol.The HPLC conditions for analyzing gallic acid and resveratrol were as follows:mobile phase:methanol(A)-water(B);detection wavelength:274 nm;gradient elution:1-15 min,5%A;15-40 min,40%A;flow rate:1.0 mL/min;column temperature:25?;injection volume:10 ?L.To optimize the extraction process of gallic acid and resveratrol,the extraction time,ultrasonic temperature,liquid to solid ratio ratio,ultrasonic power,particle size,ethanol concentration and moisture content were optimized by single factor experiments and Plackett-Burman.The optimization result showed that the ethanol concentration,liquid ratio and ultrasonic power were used for further optimization.Above three factors were optimized by response surface at the conditions of extraction time 30 min,ultrasonic temperature 40?,particle size 60-80 mesh,and moisture content 10%.The results showed that theoretical value of gallic acid and resveratrol were 0.32 mg/g and 0.082 mg/g when the liquid to solid ratio is 11:1,ethanol concentration is 76%,the ultrasonic power is 200 W.The verification test experiments proveed that the relative error of test results compared with the theoretical value is 0.63%.(3)Using macroporous resin to purify gallic acid and resveratrol.Comparing the effect of ADS-17,HPD-10OA,HPD-500,HPD-600,HPD-450 and HPD-300L on purification ofallic acid and resveratrol.HPD-500 resin was screened for purification of gallic acid and resveratrol.The best purification processes were as follows:the loading concentration of gallic acid and resveratrol were 0.31 mg/mL and 0.079 mg/mL.Adsorption temperature is o?,saturated adsorption time is 2 h,pH is 3,the salt concentration is 4%.The adsorption rate of HPD-500 to gallic acid and resveratrol were 35.69 mg/g and 51.10 mg/g,desorption temperature is 40?,desorption time is 2 h,ethanol elution is 80%,elution rate is 83.07%and 80.75%,respectively.. |
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