Anti-breast Cancer Activity And Mechanism Of Component Formula Of Prunellae Spica

Objective: To screen the active fraction of Prunellae Spica against anti-breast cancer and the mechanism behind.To evaluate the anti-breast cancer activity of Prunellae Spica In pharmacological pharmacodynamic models both in vivo and vitro to provide a preliminary evidence for further research and exploition of Prunellae Spica.Method:(1)MTT assay was applied to observe the toxicity and the anti-breast cancer activity of triterpene,polysaccharides,volatile oil fractions from Prunella Spica and their cross combination.(2)4T1 tumor cells were injected subcutaneously in right flank of BALB/c mice to establish 4T1 tumor-bearing models.Tumor-bearing mice were randomized into four groups: Control group,positive control group and drug gastric administration groups: P(polysaccharides)group,T(triterpene)group,V(volatile oil)group,PT(polysaccharides and triterpene in combination)group,PV(polysaccharides and volatile oil in combination)group,TV(triterpene and volatile oil in combination)group,PTV(polysaccharides and volatile oil and triterpene in combination)group.After 21 days administration,Micro CT,tumor-tissue HE staining immunohistochemical staining and TUNEL assays were performed to evaluate the anti-tumor activity of different fractions in 4T1 tumor-bearing female BALB/c mice.(3)Clone formation and MTT assays were applied to measure the proliferation inhibition of breast cancer cells.Hoechst33342 staining assay and flow cytometry test were applied to measure apoptosis of breast cancer cells induced by Prunellae Spica fractions.Scratch test and transwell assay were used to evaluate the inhibitory effect of PTV on MDA-MB-231 cell migration.(4)The anti-breast cancer effect of PTV and the mechanism were certified in 4T1 tumor-bearing female BALB/c mice.The tumor-bearing mice were randomized into four groups: Control group,positive control group and PTV Gastric administration groups receiving daily doses of PTV 2g,4g respectively for 21 days.HE staining and immunohistochemical staining assays were performed to evaluate the anti-tumor activity of PTV in 4T1 tumor-bearing female BALB/c mice.Result:(1)T and PTV showed a significant anti-tumor activity in vitro.(2)Both T and PTV showed good anti-tumor activities in vivo.T and PTV inhibited the angiogenesis and the proliferation of 4T1 cells,induced the apoptosis of breast cancer cells in tumor-bearing mice by downregulating the expression of PCNA.In addition,T and PTV could reduce the risk of tumor metastasis by inhibiting the expression of Ecadherin.(3)PTV could inhibit proliferation,infiltration and tumor metastasis and induce the apoptosis of MDA-MB-231 cells in vitro.(4)PTV could significantly reduce the tumor growth,shrink tumor volume and prevent from pulmonary metastasis.PTV could control tumor progression by suppressing cell proliferation,apoptosis induction and angiogenesis inhibition.Conclusion: Different fractions of Prunellae Spica in combination show a significant anti-breast cancer activity.It has a great potential in prevention and treatment of breast cancer.PTV can inhibit the proliferation of breast cancer cells and induce cell apoptosis.In addition,PTV can also inhibit the angiogenesis in solid tumor tissue therefore preventing cancer cells from migration and infiltration.PTV is worthy of further exploition as a promising anti-breast cancer drug candidate.