The Study Of Triptonide In The Treatment Of Human Pancreatic Cancer

Part One:Effect of triptonide on the proliferation? tumorigenicity and apoptosis of human pancreatic cancer cellObjectiveTo study the effects of TN on the proliferation,tumorigenicity,and apoptosis of human pancreatic cancer cell lines Patu8988 and Panc-1 in vitro.Methods1.Alamar-Blue detection reagent was used to detect the effect of TN on proliferation of Patu8988 cells and normal human fibroblasts in vitro.2.Trypan blue staining was used to observe the effect of different concentrations of TN on the tumorigenicity of Patu8988 and Panc-1 cells.3.The effect of TN on the apoptosis of pancreatic cancer cell Patu8988 was analyzed by flow cytometry.Results1.After treating Patu8988 cells and normal human fibroblasts with 0-32 n M TN for 72 hours,the growth curve showed that TN can effectively inhibit the proliferation of Patu-8988 cells in vitro in a concentration-dependent manner,while proliferation of normal human fibroblasts is relatively weak.2.Photographs with stereomicroscopes and count colony formation,the results showed that TN can completely inhibit pancreatic cancer cell colony-generating ability at a concentration of 8 n M,and present a concentration-dependent manner.3.Apoptosis assay results showed that TN caused apoptosis on the sixth day of treatment,and with the pass of time,the apoptosis rate gradually increased,with a significant time dependence.Conclusion1.TN inhibited the proliferation and colony formation of Patu8988 and Panc-1 cells in a dose-dependent manner.2.The pro-apoptotic effect is an important way of TN inhibiting the development of pancreatic cancer.Part Two:Inhibitory effect of triptonide on the growth of transplanted tumor of pancreatic cancer cell in nude miceObjectiveTo investigate the effect of TN on the growth of transplanted pancreatic cancer xenografts in nude mice and the survival of nude mice,and to explore whether TN has potential clinical anti-tumor therapeutic value.Methods1.Human pancreatic cancer cell was injected into the back of nude mice to establish a subcutaneous xenograft model.To observe the growth inhibitory effect of TN on nude mice xenografts and its effect on the growth of nude mice.2.Human pancreatic cancer cell was injected into the back of nude mice to establish a subcutaneous xenograft model.To observe the survival time of tumor-bearing nude mice after TN treatment.Results1.There was no significant difference in the body weight,heart,liver,lung,kidney and other organs index and blood cell parameters between the control group and TN treatment group.2.Transplanted tumor growth curves suggest that TN can significantly inhibit the growth of pancreatic cancer in nude mice.Of 8 TN-treated nude mice,5 of the tumors were completely inhibited by TN and the tumors of the other 3 nude mice were also significantly inhibited by TN.Statistically,there was a significant difference in the weight of transplanted tumor in nude mice between the two groups(P <0.01).3.Mice in the untreated xenograft control group survived for 53.8�21.5d on average,compared with 143.0�13.2d in mice treated with 5 mg/kg/d TN for 64 d.After statistical analysis,there was a significant difference in survival between the two groups(P <0.01).Conclusion1.TN can significantly inhibit the growth of xenografts in nude mice and prolong the survival of nude mice.2.TN does not cause severe complications at the effective anti-PC doses on the general situation of nude mice.