Chemical Constituents Of Ethyl Acetate And N-butanol Fraction From Chirita Linearifolia W.T.wang

Objective:（Chirita linearifolia W.T.Wang）is（Gesneriaceae）（Ch irita）plants,there is no literature reported its chemical research.Based on the study of the（Chirita linearifolia W.T.Wang）,the study on the c hemical composition of ethyl acetate and positronic acid was further stu died and provided a scientific basis for the research and development o f the pharmacophore.Methods:picking fresh（Chirita linearifolia W.T.Wang）medicine,under the condition of 25 degrees Celsius to dry and crush,medicinal herbs meal,three times in turn with 85%ethanol reflux extraction,50%ethanol reflux extraction,three times the total extract,total extract i n turn with petroleum ether,chloroform,ethyl acetate,n-butanol solutio n General reagent method,petroleum ether,chloroform part respectively,ethyl acetate and water area,using silica gel column chromatography,macroporous resin chromatography,polyamide chromatography and Anti-phase ODS column chromatography,MCI column chromatography,Seph adex LH-20 column chromatography methods,such as column chromato graphy and recrystallization method to its isolation and purification of e thyl acetate and n-butanol part.The structural characterization of mono mer compounds was characterized by physical and chemical examination,MS,¹H-NMR,¹³C-NMR,DEPT and other spectral methods.Results:23 monomer compounds were isolated from（Chirita linea rifolia W.T.Wang）and the structure of 18 compounds was classified.5,7,3’4’-2 hydroxy-6-c-β-D-glucose dihydroflavonone（1）1-hydroxy-2-hyd roxymethylanthraquinone（2）、R)-7-hydroxy-α-dunnione（3）、（3R）-7-Methoxy-R-dunnione（4）2-ethylanthraquinone（5）、1-hydroxy-6 methyl anthraquinone glycosides（6）,、（plantamajoside）（7）、carrot glycosides（8）、a-spinach sterol-β-D-pyran glycosidase（9）、stigmasterol（10）、and c aprolactam（11）、1-dihydroxy-2-hydroxymethyl anthraquinone（12）1-hydro xy-2-methylanthraquinone（13）、undecanoic acid（14）、Palmitoleic a cid（15）triacontanoic acid（16）、Octatriacontane（17）、2-[10（Z）-hepta decenyl]-1,4-hydroquinone（18）,the identification was isolated from（Chir italinearifolia W.T.W ang）got compounds mainly for the anthraquinones（2）and（4）（5）（6）（12）（13）nonsteroidal（7）（9）（10）,phenylethyl al cohol glycosides（7）and unsaturated fatty acids and saturated fatty acid s（14）（15）（16）and（17）alkane compounds.Conclusion:These compounds（2）（3）（5）（8）（13）for the petroleu m ether part has been compounds,（1）（2）（4）（6）（7）（9）（10）（11）（12）（14）（15）（16）（17）（18）compounds were isolated from this plant f or the first time.The（18）compounds may be new compounds that nee d further determination of the length of the carbon chain..