Chemical Constituents Of Ethyl Acetate And N-butanol Fraction From Chirita Linearifolia W.T.wang

Objective:?Chirita linearifolia W.T.Wang?is?Gesneriaceae??Ch irita?plants,there is no literature reported its chemical research.Based on the study of the?Chirita linearifolia W.T.Wang?,the study on the c hemical composition of ethyl acetate and positronic acid was further stu died and provided a scientific basis for the research and development o f the pharmacophore.Methods:picking fresh?Chirita linearifolia W.T.Wang?medicine,under the condition of 25 degrees Celsius to dry and crush,medicinal herbs meal,three times in turn with 85%ethanol reflux extraction,50%ethanol reflux extraction,three times the total extract,total extract i n turn with petroleum ether,chloroform,ethyl acetate,n-butanol solutio n General reagent method,petroleum ether,chloroform part respectively,ethyl acetate and water area,using silica gel column chromatography,macroporous resin chromatography,polyamide chromatography and Anti-phase ODS column chromatography,MCI column chromatography,Seph adex LH-20 column chromatography methods,such as column chromato graphy and recrystallization method to its isolation and purification of e thyl acetate and n-butanol part.The structural characterization of mono mer compounds was characterized by physical and chemical examination,MS,¹H-NMR,¹³C-NMR,DEPT and other spectral methods.Results:23 monomer compounds were isolated from?Chirita linea rifolia W.T.Wang?and the structure of 18 compounds was classified.5,7,3'4'-2 hydroxy-6-c-?-D-glucose dihydroflavonone?1?1-hydroxy-2-hyd roxymethylanthraquinone?2??R)-7-hydroxy-?-dunnione?3???3R?-7-Methoxy-R-dunnione?4?2-ethylanthraquinone?5??1-hydroxy-6 methyl anthraquinone glycosides?6?,??plantamajoside??7??carrot glycosides?8??a-spinach sterol-?-D-pyran glycosidase?9??stigmasterol?10??and c aprolactam?11??1-dihydroxy-2-hydroxymethyl anthraquinone?12?1-hydro xy-2-methylanthraquinone?13??undecanoic acid?14??Palmitoleic a cid?15?triacontanoic acid?16??Octatriacontane?17??2-[10?Z?-hepta decenyl]-1,4-hydroquinone?18?,the identification was isolated from?Chir italinearifolia W.T.W ang?got compounds mainly for the anthraquinones?2?and?4??5??6??12??13?nonsteroidal?7??9??10?,phenylethyl al cohol glycosides?7?and unsaturated fatty acids and saturated fatty acid s?14??15??16?and?17?alkane compounds.Conclusion:These compounds?2??3??5??8??13?for the petroleu m ether part has been compounds,?1??2??4??6??7??9??10??11??12??14??15??16??17??18?compounds were isolated from this plant f or the first time.The?18?compounds may be new compounds that nee d further determination of the length of the carbon chain..