MiRNA-204 Influence On The Invasion And Migration Of Astrocytoma Is Down-regulated By DNA Methylation

Objective:To investigate the epigenetic mechanism of tumor suppressor gene mi R-204 expression imbalance in astrocytoma and the changes of tumor cell invasion and migration.Methods: NCBI was used online to detect the position of mi R-204 in the genome,and bioinformatics software is used to analyze whether there is methylation in the mi R-204 promoter region.DNA methylation status of mi R-204 gene promoter in 2 normal brain tissues and 4 astrocytoma tissues were detected by BSP and MSP.Expression of mi R-204 in 10 normal brain tissues and 30 astrocytoma tissues was detected by expanded sample q PCR.The astrocytoma cell line was treated with demethylated drugs-5-Aza-d C and mi R-204 si RNA to observe the invasion and migration ability of the cell line.Results: There are typical cpg islands in the mi R-204 promoter region,located in 1703-1944 region,with a length of 242 bp,compared with the normal tissues,the promoter region of mi R-204 gene was relatively hypermethylated,and the corresponding m RNA expression was low in tumor tissues.The difference was statistically significant(P < 0.05).The role of mi R-204 as a tumor suppressor gene also inhibited the invasion and migration of astrocytoma,while the increase of DNA methylation increased the ability of invasion and migration of astrocytoma.We inhibit the invasion and migration of astrocytoma by reducing the degree of DNA methylation in the mi R-204 promoter region.There is a negative correlation between the degree of DNA methylation and the degree of DNA methylation.Conclusion: In the tissue of astrocytoma,the promoter region of mi R-204 gene is relatively high methylation,and the transcriptional activity of mi R-204 is significantly reduced.Its expression imbalance was closely related to DNA methylation in its promoter region.Its correlation and its mechanism may be regulated by ezrin protein,which needs further study.