Relationship Between Postmenopausal Osteoporosis And Immune Function By High-Throughput Sequencing

Objective To detect the relationship between postmenopausal osteoporosis and immune function.We detected the differentially expressed genes between postmenopausal osteoporosis group and non-osteoporosis group,and carried out functional enrichment analysis of these differentially expressed genes,and studied the signal pathway to find the relationship between postmenopausal osteoporosis and immune function.Methods The study involved 6 women including 3 postmenopausal osteoporosis and 3 postmenopausal non-osteoporotic women.The bone mineral density(BMD)can be assessed with dual X-ray absorptiometry(DXA),and osteoporosis is defined by a T-score-2.5 or more standard deviations below the average of a young adult.The control group was is defined by a T-score>-1 standard deviation.After an overnight fast,morning blood was drawn from the candidates.Total RNA was extracted from peripheral blood mononuclear cells.RNA quality and purity was confirmed with a Nanodrop spectrophotometer and electrophoresis.RNA library was constructed.With Illumina Hiseq 2000 sequencing platform,we collected dates and searched for differentially expressed genes(DEGs).Afterwards,functional and pathway analyses were conducted.Results Our results showed that 108 differentially expressed genes were found in the experimental group(postmenopausal osteoporosis patients)and the control group(postmenopausal non-osteoporotic patients),including 56 up-regulated genes,52 down-regulated genes.The differentially expressed genes were mainly enriched in GO term of defense response,Immun response,inflammatory response,lymphocyte activation,leukocyte activation.The results suggested that postmenopausal osteoporosis and the patient's immune function are closely related.Increasing the immune function of the patients may be a potential therapeutic target for postmenopausal osteoporosis.Conclision The results indicated that postmenopausal osteoporosis was associated with changes of immune function.