The Analysis On Human Oral Microbiome Related With Health And Disease By High-throughput Sequencing

Objective: By using high-throughput sequencing, we selected people as subjects who with dental caries, periodontitis and without oral disease, analyzed their salivary microorganisms,explored different pathogenic mechanisms of the microbial community between caries,periodontitis or healthy mouth, and provided laboratory evidence for the prevention and treatment of oral diseases.Methods: According to the WHO sampling standard, 30 subjects were collected and divided them into caries(C) group, periodontitis(P) group and health(H) group. Their salivary DNA were extracted, then the 16 S r DNA V4 region wre amplified by PCR and sequenced by MiSeq Illumina, the microbial diversity and structure analysis were analyzed by Qiime, Mothur,Megan, SPSS and other software.Results: 1. A total of 1046647 high-quality sequences were obtained from 30 samples, the average length of read was 223bp;2. 17796 OTUs were measured and belonged to 28 phyla(group H: 26, group C: 24, group P:25) and 506 genera(group H: 390, group C: 357, group P: 378). Some of them, which the prevalence is more than 1%, were called dominant phyla(H Group: 7, group C: 7, group P: 9) and dominant genera(group H: 36, group C:33, group P: 34);3. In the dominant genera, Actinomyces, Rothia, Streptococcus, Neisseria and Actinobacillus have a common existence in each group.4. When compared with group H, the abundance of Actinobacteria, TM7 bacterial phylum was higher in group P, but that of the Bacteroidetes was lower at bacterial phylum level; while that of the Rothia, Megasphaera was higher in group P, but that of the Abiotrophia, Granulicatella and Lactococcus was lower at bacterial genus level(P < 0.05);5. The dominant bacterial phyla which had significant difference were Actinomycetes and TM7 bacterial phylum in both group C and group P, with the abundance higher in group P than in group C; while the dominant bacterial genera were Gemella and Granulicatella, with the abundance in group C higher than that in group P(P < 0.05).Conclusion:1. There were certain differences of the microbial structures in saliva of three groups, and the bacterial flora of the disease groups are relatively simple;2. The abundance of some bacterial phyla or genera were positively correlated with themorbidity of periodontitis or caries;3.The core microorganisms in the health group, caries group and periodontitis group were all existed, there are core microbes present in oral.