Expression And Significance Of CD3?CD4?CD11b And CD45 In Conjunctival Epithelial Cells Of Patients With Diabetes Mellitus

Objective:To investigate expression and the mechanism of CD3,CD4,CD11b and CD45 in conjunctival epithelial cells of patients with diabetes mellitus.Methods:A total of 49 patients with normal type 2 DM and normal subjects were randomly selected from the control group(27 eyes)and the DM group(22 eyes).The right eye was selected as the experimental eye.The ocular surface disease(BUT),basal tear secretion test(schirmer I-test)and corneal fluorescence staining were used to detect the expression of CD3,CD4,CD4(superscript+)in conjunctival epithelial cells by flow cytometry(FCM)CD11b and CD45 and the ratio of CCR4+CD4+,CCR5+CD4+and CCR6+CD4+cells.Results:1.The OSDI score(26.23 ± 9.65)in DM group was higher than that in normal group(9.52± 8.67),and there was significant difference(P<0.01).2.Corneal fluorescein staining score,BUT test and Schirmer I test compared with the control group(2.41 ± 2.13 points,12.17 ± 2.31s,15.21 ± 3.25mm)compared DM group(3.56± 1.93 points,3.64±1.23s,7.16±1.09 Mm)were statistically different(P<0.05,P<0.01).3.The expression of CD4±in DM group was significantly higher than that in normal group(10.45±2.02,2.47±0.49).There was no significant difference between CD3,CD11b and CD45 in the two groups(P<0.05).4.The percentage of CCR5+CD4+,CCR6+CD4+cells in the DM group was significantly higher than that in the control group(2.41±1.94%,4.89±1.90%)(P<0.05)The ratio of CCR4+CD4+cells was not statistically different.Conclusion:1.conjunctival epithelial regulatory T cells may be involved in the pathogenesis of DM dry eye;2.Detection of chemokines CCR5+CD4+and CCR6+CD4+in conjunctival epithelial cells by flow cytometry can be used as an auxiliary examination item and diagnostic index for judging tear film function.