The Study Of The Expression Of Regulatory T Cells In Patients With ROU

Objective:To discuss the function of regulatory T cells and correlated cytokine in the occurrence and development of ROU, and to clarify the mechanism of ROU, through detecting the expression of them in the peripheral blood of ROU patients, aiming at providing more effective treatment for ROU patients.Methods:There were21patients with ROU in experimental group and21healthy people in control group. The sex and age were well matched between the two groups.3ml peripheral blood was extracted for all people,2ml peripheral blood was used to detect the ratio of CD4+CD25+Foxp3+Treg cells by Flow Cytometry. lml frozen peripheral blood was used to detect the level of IL-17and IL-2by ELISA.Statistical analysis was performed using SPSS13.0software package. Continuous variables were presented as mean±standard deviation(SD), two-sample T-test was performed between two groups. Correlation analysis was used in the ulcer group between CD4+CD25+Foxp3+Treg cells and IL-17.Results:1. The results of Flow Cytometry test:The portion of CD4+CD25+Foxp3+T cells was lower in the patients with ROU than those in the control group, and there was significant difference between two groups (P<0.05).2. The expression of cytokine detected by ELISA:The expression of IL-17was higher in patients with ROU than those in the control group, and there was different in statistics (P<0.05). The level of IL-2was lower in the patients with ROU than those in the control group, and there was significant difference between two groups (P<0.05).3. The correlation between CD4+CD25+Foxp3+Treg/CD4+T and IL-17in ROU patient.CD4+CD25+Foxp3+Treg cells correlated negatively with the level o f IL-17in patients with ROU(r=-0.582, P<0.05). Conclusions:1. Regulatory T cells was lower expressed in the peripheral blood of ROU patients compared with control group, and played an important role in the development of the disease.2. The higher expression of IL-17in the period of ROU, which can aggravate the progress of the disease, played an inflammation-causing effect in the genesis and development of ROU.3. The reduction of IL-2in peripheral blood of patients with ROU ulcers results in abnormal immune function, declined the ability to resist pathogenic microbes and results in ROU.4. The imbalance in the expression of regulatory T cells and IL-17in the peripheral blood of ROU patients may play an important role in the development of ROU.5. The study of the expression of regulatory T cells and correlated cytokine in ROU patients was helpful for clinic to find cause of disease, diagnosis and treatment more effectively.