Euscaphis Konishii Active Ingredients And Inhibitory Effect On Cancer Cells Research

Euscaphis konishii is staphyleaceae Euscaphis plant,also called "Ji Zhun Zi","Ji Yan Jing"or"Gou Tou Jiao".As a kind of traditional Chinese herbal medicine in China,it has a long history and good pharmaceutical ground.It is widely used in stomach pain,traumatic injury,rheumatic pain,dizziness,diarrhea and dysentery.According to reports,three kinds of esters by separation and purification from Euscaphis japonica leaf extract,found Euscaphis ester compounds can inhibit the malignant proliferation of HeLa cells,and promote apoptosis of tumor cells.In this paper,extracted of the Euscaphis konishii leaves and branches collected in winter and summer by using ethanol as extraction solvent,then extracted the crude extracts by organic solvents,petroleum ether,ethyl acetate,butanol extraction of,and collect extract components,carries out the following research:1.Identified the physicochemical properties of the extracts,determined the classification of its contents.2.Extraction of inhibition on the liver cancer cells in vitro pharmacological screening experiment,three stage conclusions were:(1)In vitro,the inhibiting rate of extract on human hepatoma cells BEL-7404 from ethyl acetate phase was the best.(2)Based on the contrast experiments,on inhibition of hepatocarcinoma cell,the ethyl acetate extracts of leaves and twigs that collected in winter t was better than the summer's.(3)The inhibition rate of ethyl acetate extracts of twigs on hepatoma cells was better than leaves collected in winter.3.By thin layer chromatography,silica gel column chromatography and preparative chromatography in high pressure,two compounds A and B were found,Identified the structures of the two compounds using IR,MS and NMR,compound A was Ethyl iso-allocholate,B was a new compound,the two compounds were separated for the first time from this plant.The results of the inhibitory rate on BEL-7404 hepatocellular carcinoma cells showed that compounds A and B had good effects,the IC50 of A was 501?19mg/L,B was 332.66mg/L.And compound A and B to BEL-7404 in vitro cell inhibition rate was 58.9%,58.5%.This study provided the basic data for the research of Euscaphis konishii active ingredients of secondary metabolites,broaded the research direction,laid the foundation for the further study on the Euscaphis konishii.