Apoptotic Effects Of Total Flavonoids From The Leaves Of Carya Cathayensis Sarg.on U266 And Related Protein Expression

Popupose To Select Flavonoids from the Leaves of Carya cathayensis Sarg(Total Flavonoids,chrysin,Pinostrobin chalcone,pinocembrin,wogonin,cardamonin)an effective drug and effective concentration to inhibit U266 cell proliferation,To observe the apoptotic effects of flavonoids from the leaves of Carya cathayensis Sarg.on U266 and their underlying mechanisms.Studying the flavonoids from the leaves of Carya cathayensis Sarg.affect related apoptosis protein expression about U266.Methods ?Drug Selecting,we observed the proliferation changes after the lavonoids from the leaves of Carya cathayensis Sarg.(hickory leaf flavonoids,chrysin,radix scutellariae,pinocembrin,han chalcone,cardamom)influence on U266 cells by ordinary microscope,screening effective drugs by counting Grow inhibitory rate.? To observeTotal Flavonoids from the Leaves of Carya cathayensis Sarg.U266 cells after 24 hours adopted to the CCK 8 method,screening U266 cells growth inhibition rate,screening the effective drug concentration.? to observe flavonoids from the leaves of Carya cathayensis Sarg.effects U266 cells morphological changes of apoptosis cells after 24 hours by the ordinary light microscope and fluorescence microscope(AO/PI staining).? to test flavonoids from the leaves of Carya cathayensis Sarg.action U266 cell apoptosis rate after 24 hours by flow cytometry.? to detect PTEN+P13K/AKT signaling pathways of apoptosis related proteins in flavonoids from the leaves of Carya athayensis Sarg effects U266 cells after 24 hours by the WesternBlotting technologyResults ?Total flavonoids from the leaves of Carya cathayensis Sarg.and cardamonin can inhibit U266 cells proliferation obviously,but we detect crystal in the high concentrations cardamonin,the cardamonin inhibition rate of low concentration is lower than Total flavonoids from the leaves of Carya cathayensis Sarg.?results of the CCK 8 suggests Total flavonoids from the leaves of Carya cathayensis Sarg can inhibit U266 cell proliferation,and a certain amount of activity relationship,theIC50 of U266 cell proliferation is 39.92ug/mL.? we observed the typical morphological changes of apoptosis through AO/PI staining cells by fluorescence microscopy.? results of flow cytometry show that the cell apoptosis rate pecan U266 cell apoptosis can be induced by leaf flavonoids,dependency and concentration-effect.? WesternBlotting results show that Total flavonoids from the leaves of Carya cathayensis Sarg down regulation apoptosis gene P-AKT of U266,PTEN protein expression of PTEN protein increases.Conclusion Total flavonoids from the leaves of Carya cathayensis Sarg can through up-regulation the PTEN genes and down the P-AKT genes expression to refrain cell proliferation,and can suppress cells growth with concentration-effect relationship.