Expression Changes Of BFGF And TGF-β1 In Extraocular Muscles Of Cat Model With Exotropia

Objective The goal of this study was to observe the expression changes of Basic fibroblast growth factor(bFGF)and Transforming growth factor-β1(TGF-β1)in medial rectus muscle of cat model with strabismus.Method We selected 27 little cats,which were in sensitive period(4-6w),through surgical methods to product exotropia model.All animals were randomly divided into experimental,controla and normal groups.Collected medial rectus muscles of the experimental、control and normal groups,respectively,after strabismus surgery a month,2 months,3 months.These collection were prepared for histologic examination by fixation,paraffin embedding and slice.By Immunohistochemistry staining to detect bFGF and TGF-β1 expression and by optical density analysis method to measure the average optical density value.Results ① The expression of the bFGF in the medial rectus muscle is increased.There is a significant difference(P<0.05)of the bFGF expression in the medial rectus muscle between the after three months group with the control group as same as normal group.Then there are no obvious differences(P>0.05)of bFGF expression in the other two groups.②The expression of TGF-β1 in the medial rectus muscle is increased.There are significant differences(P<0.05)of TGF-β1 in the medial rectus muscle between the three experimental groups with control groups as same as normal groups.③The expression of bFGF and TGF-β1 is increased with the duration of the extension.Conclusion ① The surgical method can be successfully produced concomitant exotropia cat model.② The increased expression of the bFGF and TGF-β1 in extraocular muscles may be involved in the occurrence of strabismus.③There is positive relationships between bFGF expression with Strabismus course of the disease as same as TGF-β1.