Research On The Correlation Between SLC30A8 Gene Polymorphism And Type 2 Diabetes Mellitus In Gansu Han Population

Objectives:To study the genotype distribution of nucleotide polymorphisms(SNPs)rs13266634、rs11558471 in SLC30A8 gene in gansu area.To investigate the association of SLC30A8 gene polymorphism with type 2 diabetes mellitus in Gansu Han population.Methods:Randomly selected 116 cases of type 2 diabetes mellitus patients from Gansu Provincial,Department of Endocrinology and The first people’s hospital of Lanzhou city,Department of Endocrinology,hospitalization.Besides,80 normal cases selected from Lanzhou City People’s Hospital Health Examination Center,normal cases has no family history of diabetes and cases of type 2 diabetes mellitus patients were excluded from type 1 diabetes patients、combined with acute complications(ketoacidosis,hyperosmolar state),merger severe liver and kidney dysfunction,associated with malignant tumors in patients.DNA extraction kit are used to extract DNA,polymerase chain reaction(PCR)are used to amplify the target DNA.The polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)are used to detection SLC30A8 gene polymorphism.At the same time,we measured the height,weight,waist,hip,automatic biochemical analyzer plasma total cholesterol are used to detect triglycerides,low-density lipoprotein cholesterol,high-density lipoprotein cholesterol,fasting glucose,fasting insulin was measured by radioimmunoassay;using stable state model assessment of pancreatic B cell function index(Homa-B)and insulin resistance index(Homa-IR):Homa-B = 20X fasting insulin(mU/L)/(FPG-3.5);Homa-IR = fasting insulin(mU/L)x fasting plasma glucose(mmol/L)/22.5.Microsoft Office Excel 2003 to establish a database,SPSS 13.0 software is used for statistical analysis.Results:1.Compared with normal group,the two groups was not statistically significant in weight and BMI(p>0.05);but the WC and WHR of T2DM group are significantly higher than the NGT group(respectively,P<0.01,P<0.05);the T2DM group fasting plasma glucose(FPG),low-density lipoprotein cholesterol(LDL)were significantly higher than those in NGT group(P<0.01);the NGT group homeostasis model assessment islet B cell functionindex(Homa-B),high-density lipoprotein cholesterol(HDL),fasting insulin(FINS)were significantly higher in T2DM group(P<0.01).Compared with NGT group,insulin resistance index Homa-IR,total cholesterol(TC),triglyceride(TG),diastolic blood pressure(DBP),uric acid(UA)was no statistically significance of T2DM group(P>0.05);2.(1)The frequency of SLC30A8rs13266634 CC,CT,TT genotype was 29.6%,41.3%and 29.1%in the study population.The CC genotype of T2DM group was significantly higher than the NGT group,while the TT genotype was much belower than NGT group.C risk allele frequency in T2DM group was 59.1%,significantly higher than 37.5%of the NGT group.Compared with CC genotype of T2DM group,the frequency of TT genotype frequency was statistically significant(P<0.01),C-risk allele T allele 2.404 times the risk of suffering from T2DM(OR = 2.404,95%CI:1.590-3.634,2 = 17.594,P = 0.000).The difference was statistically significant(P<0.01);(2)CC genotype FINS,Homa-B was significantly lower than the TT genotype(P<0.05).CC genotype SBP,TQ UA below the TT genotype was statistically significant(P<0.05).Between the three genotypes DBP,FPQ TC,HDL,LDL,Homa-IR,BUN was no significant difference(P>0.05).3.(1)The frequency of SLC30A8rs 11558471 AA,AG,GG genotype in present study was 32.3%,52.1%and 15.6%,respectively.The frequency of T2DM group AA genotype was much higher than the NGT group,while the frequency of GG genotype was less than the NGT group.A risk allele frequency T2DM group was 61.0%,which much higher than the NGT group,54.5%T2DM group AA genotype frequency of GG genotype frequency was no statistically significance(P>0.05).A risk allele risk of T2DM in G allele 1.307 times(OR = 1.307,95%Cl:0.839-2.036,2 = 1.405,P = 0.236).(2).AA genotype AA genotype SBP,TG,FPG,BUN,UA was significantly higher than the GG genotype(P<0.05),while the AA genotype FINS,Homa-B,Homa-IR was significantly lower than the GG genotype(P<0.05).The AA genotype Homa-IR below the AG genotype(P<0.05),AA genotype Homa-B,BUN,UA than GG genotype(P = 0.05).4.Compared with normal body group,the frequency of SLC30A8rs13266634 CC,CT and TT genotype were 27.9%,42.6%,29.5%in overweight and obese group.Comparison,overweight and obesity CC,CT and TT genotype frequencies were 27.5%,56.9%,15.7%,and there have no significant difference.C allele relative to the T allele of the obesity/overweight risk does not have statistical significance(OR=1.309,95%CI:0.772-2.219,χ2=1.000,P=0.317).Conclusion:1.There was a closely relationship between the SLC30A8 gene rs13266634 polymorphism and type 2 diabetes in Gansu province;and the C allele of SLC30A8rs 13266634 may be the risk allele gene in T2DM.2.The rs11558471 polymorphism of SLC30A8 gene are exist in Gansu Han population.But there was no significant correlation of type 2 diabetes in Gansu province.3.SLC30A8 gene polymorphism are associated with fasting insulin and levels islet B cell function but not insulin resistance.4.Compared with normal group,there are still elevated blood sugar,blood pressure,blood lipids,blood uric acid in patients with type 2 diabetes who has treated before.