Preparation And Biomedicine Application Of PS Nanoparticles With Surface Functionalization

Objective:The styrene and acrylic acid were used as comonomers,carboxylation polystyrene(PS-COOH)nanoparticles were fabricated by emulsion polymerization.Then the nanoparticles were applied as the carrier of Latex-enhanced Immunoturbidimetry Assay(LETIA)and capillary electrochromatography(ECT).Methods:Utilize St and acrylic acid as comonomers,potassium persulfate(KPS)as the initiator,sodium dodecyl sulfate(SDS)as the surfactant,and the PS-COOH polymer nanoparticles was fabricated by emulsion polymerization.The influences of concentrations of SDS were investigated.The nanoparticles were characterized by Scanning Electron Microscope,Infrared Spectrum analysis,Micro-particular analysis,etc.The carboxyl on the surface of the PS-COOH microspheres was activated by N-hydroxysuifosuccinimide(Sulfo-NHS)and 1-(3-Dimethylaminopropyl)-3-ethylc-arbodiimide hydrochloride(EDC),and then coupled with ?2-M to preparate?2-M-LETIA testing reagent.The influences of the concentrations of Sulfo-NHS,EDC,antibody,and the particle size,the density of carboxy of PS-COOH,etc.on the performance of ?2-M-LETIA were investigated.Self-manufactured samples were measured by UV and the Automatic Biochemistry Analyzer.Finally,the testing results were manipulated and analysised by statistic means.After activated by EDC and Sulfo-NHS,BSA was coupled on PS-COOH nanoparticles in MES solution,and the PS-COOH/BSA nanoparticles were fabricated.Then,PS-COOH/BSA microspheres were immobilized onto a capillary innerwall followed the four main steps.First,the pretreatment of the capillary column.Next,amination of the capillary innerwall.Then,embellishment of the capillary innerwall with formyl group.Finally,immobilizetion of PS-COOH/BSA nanoparticles.The performance of the capillary modificated by PS-COOH/BSA nanoparitiles were investigated by the application of chiral separation of tryptophan.Results:The nano-sized PS-COOH microsphere has been managed by emulsion polymerization methods.Via adjusting and controlling of the preparation conditions,the size of the microsphere has been controlled within a certain range.By means ofscanning electron microscope,the nanoparticles have a good degree of sphericility and an uniformitarian size distribution;Carboxyl groups of PS-COOH naoparitcles is proved by the infrared spectrum.The optimum conditions of coupling ?2-M-antibodies to the PS-COOH microspheres have been obtained and the ?2-M-LETIA reagent is prepared.Then the self-manufactured ?2-M-LETIA reagent,were successfully applied to detect the samples and the good result were obtained in a certain limitation.In this work,PS-COOH/BSA nanoparticles were prepared and chemically immobilized onto the capillary innerwall,and characterized.The capillary column coated by PS-COOH/BSA could be used for chiral separation of D,L-tryptophan,the resolution and the reproducibility of the result were good.Conclusion:The size-controlled and monodispered PS-COOH microsphere are fabricated.Development of the method of coupling ?2-M antibodies on PS-COOH nanoparticles and the ?2-M-LETIA reagent is fabricated.After optimization of the coupling conditions,the optimum conditions are obtained.Moreover,the resolution of the nanoparticle-immobilized capillary was significantly improve compared with that of monolayer BSA-modified capillary.Meanwhile,the resolution was increased with the decrease of the diameter of the nanoparticle.