Preparation And Application Of Functional Polymer Nanoparticles In Biology And Medicine

Objective: Poly glycidyl methacrylate(PGMA) organic polymer microspheres were prepared, and the effect of different reaction temperature, speed, monomer and initiator concentration, the amount of emulsifier, the amount of crosslinking agent on PGMA microspheres particle size and polydispersity index(PDI) were detected. Then the method for chemically immobilization of PGMA nanoparticles(NPs) onto a capillary inner wall was developed in order to improve the specific surface area of capillary. The prepared NPs-coated capillary was finally used for open tubular capillary electrochromatography(OTCEC).The new method of preparation of magnetic nanoparticles(MNPs) was explored, and the surface of MNPs were modified by antibody using a click-chemistry reaction. The magnetic separation performance of immune magnetic microspheres with the corresponding antigen of tumor cells were explored in order to develop a novel tumor-targeted immuno-reagents. The methods of synthesis and purification of the azide benzoyl hydrazine were investigated. Methods: Using potassium persulfate(KPS) as initiator, sodium dodecyl sulfate(SDS) as emulsifier, ethyleneglycol dimethacrylate(EDMA) as crosslinking agent and glycidyl methacrylate(GMA) as polymerization monomer, PGMA nanoparticles were prepared by emulsion polymerization method. The Beckman Delsa Nano Particle Analyzer and Scaning Electron Microscopy(SEM) were used to characterize the PGMA NPs. The immobilization of PGMA NPs onto the capillary was obtained by a ring-opening reaction between the NPs and an amino-silylated fused capillary inner surface. SEM images clearly showed that the NPs were attacted to the capillary inner surface in a dense monolayer. After lysine(Lys) coupled onto the PGMA NPs, the capillary was applied to separate the mixture of three amino acids. Run-to-run and column-to-column reproducibilities in the separation of the amino acids using the NPs-modified column were also demonstrated.The monodisperse MNPs were prepared by the solvent thermal method. Effect of reaction temperature, reaction time, sodium acrylate/sodium acetate was investigated in order to obtain the optimal reaction conditions. Infrared spectrum(IR), transmission electron microscopy(TEM) and X-ray diffraction(XRD) were used for characterization of the MNPs. The oriented immobilization of antibody onto MNPs was performed by a click-chemistry reaction. The prepared MNPs/IgG were applied for the magnetical separation of tumor cells and the result was also compared with MNPs/IgG obtained by random antibody immobilization method. The azide benzoyl hydrazine was compound by azidobenzoic acid and hydrazine hydrate. Results: PGMA NPs were prepared by emulsion polymerization. The control of particle size was realized through optimizing the preparation condition. SEM images show the sphericity and monodispersity of PGMA. The PGMA NPs coated capillary column was prepared, the PGMA NPs coated capillary column used for the separation of amino acids mixture. The synthesis of MNPs was explored. IR shows that the success of antibody directly immobilized onto the surface of MNPs. The result of tumor cell separation test indicated that the method of oriented antibody immobilization is better than that the method of random antibody immobilization. Conclusion: PGMA NPs prepared by emulsion polymerization has a narrow size distribution and good degree of sphericility. Through optimizing the preparation conditions, the partical size could be controlled. The PGMA NPs coated capillary column was prepared successfully.Then the application result of the separation of amino acids mixture proved that the prepared capillary column has excellent separation preformance and reproducibility. The monodisperse MNPs were successfully prepared, and the antibody was immobilized on the surface of MNPs using the method of click chemistry. The results show that the MNPs modified by the method of oriented antibody immobilization has higher clearance of tumor cells compared with those modified by the method of random antibody immobilization. The azide benzoyl hydrazine was synthesised by azidobenzoic acid and hydrazine hydrate, and the methods of synthesis and purification were optimized.