Study On Traditional Chinese Medicine Syndrome Patterns And Distribution Of Glucocorticoid-induced Osteoporosis And Its Characteristics In Rats

ObjectiveTo study the TCM syndrome patterns and distribution of Glucocorticoid-induced Osteoporosis.MethodsCollected in September 1,2012 to December 31,2014 hospitalized in our hospital of glucocorticoid-induced osteoporosis(GIOP)patients medical records,with reference to the traditional Chinese medicine syndrome types related standards,based on clinical manifestations of patients,patients with each assigned to the different type and gathering receipt.ResultsCollected in our hospital over the past 28 months meets the requirements of the number of cases,a total of 242 patients in hospital,the doctor of traditional Chinese medicine syndrome type distribution of kidney Yang deficiency respectively in 127 cases,61 cases of kidney Yin deficiency syndrome,spleen deficiency syndrome 31 cases,liver depression and qi stagnation syndrome,16 cases of blood stasis bizu 7 cases,accounted for 52.48%of the total number,25.21%,12.81%,6.61%,12.81%.ConclusionGlucocorticoid-induced osteoporosis of the TCM syndrome type distribution is given priority to with kidney deficiency,with a high incidence of kidney Yang deficiency syndrome.Objective To study the difference in the bone mass and the level of bone turnover markers and Estrogen,and the mechanism through the Osteogenesis and adipogenesis pathway between the process of osteoporosis induced by two kinds of glucocorticoid Methods 46 female aged 3 months SD rat were divided randomly to 4 groups as below,Baseline group(BL group,6 rats),Age-matched control group(CON group,14 rats),Prednisolone-treated group(PRE group,14 rats),Dexamethasone-treated group(DXM group,14 rats).BL group were euthanized at the beginning of the experiment.PRE group and DXM group were injected with PRE in a dose of 5mg/kg per day and DXM in a dose of 1mg/kg twice per week,respectively during 3 months.CON,PRE,DXM rats were euthanized in one month(Ml),two month(M2)and three month(M3)post-treatment,uterus and adrenal were collected for weight calculation,serum were collected for the test of estrogen,PINP and β-CTX,lumber 1-3 were isolated for bone mineral density(BMD).The bone marrow in right femur was collected for Oil red staining to observe the Cell types,lumber 6 was collected for testing the activity of OPG and PPAR-γ by RT-PCR,lumber 1-3 were collected for BMD at the end of treatment.Results BMD in PRE rats and DXM rats was lower than CON rats at each time point(P<0.05),which decreased more notably in DXM rats(P<0.01),moreover,BMD in-DXM rats decreased significantly compared with PRE rats at M2 and M3(M2:P<0.05;M3:<0.01).With regarded to the level of estrogen,although estrogen level in PRE rats was lower than CON rats at M1(P<0.01),it increased to the similar level of CON rats at M3(P>0.05),while estrogen in DXM rats sustained in the lower level at each time point compared with CON rats(P<0.05).As for the level of PINP and β-CTX,the level of them in two glucocorticoid-treated groups were significantly higher than CON rats at each point(P<0.01),additionally,the level of PINP in DEX rats was lower than PRE rats(P<0.05).Moreover,DXM rats decreased significantly compared with PRE rats in BMD.Although the expression level of PPAR-γ were no significant difference among 4 groups,OPG in DXM rats was lower than other groups.The amount of red-staining cell in two glucocorticoid treated rats were more than BL,but there was no obvious difference between the treated groups.Conclusion The effect of DXM on bone mass decrease was more powerful than PRE,which might be caused by more notable shrinkage of estrogen secretion and bone formation activity,as well as might be caused by restraining the expression of OPG more significantly through OPG/RANKL/RANK pathway.