The Research On Cell-free DNA In The Early Diagnosis And Reperfusion In Acute Myocardial Infarction

Objectives:The aim of the present study was to investigate hourly concentration of cell-free DNA changes following an acute ST-elevation myocardial infarction(ASTEMI)and to assess any correlations with vascular recanalization by percutaneous transluminal coronary angioplasty(PTC A).Methods:Serial cell-free DNA level were determined by quantitative Real-time PCR in 28 ASTEMI patients and once in 50 healthy individuals.Concentrations of cell-free DNA in patients were compared with healthy subject and the same comparation between before under going PTCA and after.And receiver operating characteristic(ROC)curves were used to analyse diagnosis value of cell-free DNA in acute myocardial infarction.Results:Cell-free DNA concentrations in patients with ASTEMI showed a significant elevation compared with healthy controls(Lg(mean�SD):(3.88�0.78)vs.(2.39�0.85),P<0.05).And level of cell-free DNA after undergoing PTCA decreased significantly than before(mean�SD:(48.78�34.15)vs.(78.83�46.66),P<0.05).But the cardiac troponin I remains elevating.ROC curve indicated that area under the curve is 0.870(P<0.05)and asymptotc 95%confidence interval ranged 0.764 to 0.976.Conclusions:Cell-free DNA may be a valuable marker for early diagnosing and have a potential clinical value for assessing the flow in the reperfused myocardium and monitoring the progress in ASTEMI.