Research On Anti-nocardia Of Traditional Chinese Herbal Compound DS

Objective:Screening anti-Nocardia effective Chinese herbal compound?DS?,the preparation of Chinese medicine compound DS best extract,to provide reference for the research and preparation of Chinese herbal extracts against Nocardia.Methods:?1?Determination of Nocardia suspension concentration and absorbance,determine the relationship between them,the establishment of Nocardia suspension quantitative solution in Nocardia.Using the broth dilution method,enrofloxacin solution using different concentrations of Nocardia MIC values and MBC values of minimum inhibitory concentration and minimum bactericidal concentration as the index evaluation method for determination of in vitro inhibition and killing effect of Nocardia,screening the anti-Nocardia best effect,MIC value is the minimum inhibitory concentration and minimum the MBC value is the lowest minimum bactericidal concentration of compound DS.?2?The evaluation method using UV vis the establishment of Chinese medicine DS compound chemical spectrophotometry method,effects of compound DS with different extraction methods,heating reflux extraction of extraction solvent,refluxing extraction of grinding degree of material,refluxing extraction solvent dosage,extraction time and heating reflux heating reflux extraction times;the use of L₉?3⁴?orthogonal test of reflux extraction,the number of level factors of reflux extraction of medicinal powder and heating time,heating reflux extraction,the amount of solvent refluxing extraction reflux extraction,the optimum reflux extraction best refluxing extraction technology of compound Chinese medicine DS.?3?Using the petroleum ether-ethyl acetate?8:2?as the expansion agent,the TLC was used for the qualitative identification of the best extracts of the Chinese medicine compound,DS,C.Using a mobile phase of methanol-water?80:20?,using Eclipse XDB C₁₈8 chromatography column?4.6mm×150mm,5mm?,column temperature is 25?,detection wavelength of 270nm,using DAD detector,analysis time is 30min,the use rate was 1.0ml/min,C content index of component preparation the Chinese herbal compound 1g/ml DS best extract chromatography which was determined by the HPLC method using high performance liquid.?4?Comparison of intraperitoneal injection sites,wound surface soaking,soaking,feeding and non trauma infection with different methods,the infection rate of animal,animal mortality,Nocardia examination as the evaluation index,the establishment of Nocardia infection animal model.To establish the animal model of Nocardia infection based on body symptoms,anatomy,visceral Nocardia examination,mortality,survival quality index,the comprehensive evaluation index of different dosage on the treatment effect of animal.?5?The three representative immune indexes,including lysozyme,alanine aminotransferase and alkaline phosphatase,were set up in the drug delivery group,the infection control group and the blank group.The changes and their changing rules in the 12D period were compared.Results:Through the screening effect of anti-Nocardia is the best medicine DS compound.The UV maximum absorption wavelength of the DS extract of Chinese medicine compound was 270nm,and the methods of linear regression,stability,recovery rate and reproducibility were all less than 2%.The preparation process of the best extract of Chinese herbal compound DS is:crushing the 60 mesh,heating the reflux and selecting solvent 5,heating and refluxing for 3 times.The time of reflux extraction is 45min,and the amount of solvent used for heating reflux is 8 times.The indicator spots of thin layer identification samples and reference materials of DS extract of Chinese herbal compound showed at the same level of height,showing clear in natural light,254nm and 366nm ultraviolet lamps,and the calculated Rf value was suitable.The use of 2×10⁸CFU/ml Nocardia suspension by using intraperitoneal injection to establish the site of infection animal model of infection symptoms significantly,mortality rate of intraperitoneal injection site infection was10%,intraperitoneal injection site infection rate was 100%,good repeatability.The compound DS the best extract in the treatment of experimental animal Nocardiosis for continuous 2D 20%feed feed,adding 20%dosage of 30%Chinese herbal extract DS best mixed feed,the experimental animal after normal feeding?80%?residual feed,the cure rate reached 100%,no abnormal pathological observation of 12D during the period of experimental animal.The best extract of Chinese herbal compound DS has the effect of increasing lysozyme,stabilizing glutamic pyruvic transaminase and increasing alkaline phosphatase activity,and has the effect of improving animal immunity.Conclusion:The compound DS best extract against Nocardia had the best effect,provide anti-Nocardia reference to research on the exploitation of traditional Chinese medicine.