The Effect And Mechanism Of CircPRKD3 On Proliferation Of Prostate Cancer Cells

Object: Elucidate the role and mechanism of circPRKD3 in inhibiting prostate cancer cell proliferation.Methods:1.Prostate cancer and benign prostatic hyperplasia tissue samples were collected for histological examination,and differentially expressed circular RNAs were selected for further experiments.2.The expression levels of circPRKD3 in prostate cancer and benign prostatic hyperplasia tissues were detected by RT-PCR,and the expression levels of circPRKD3 in prostate cancer and benign prostatic hyperplasia were compared at the cellular level by immunofluorescence staining in situ hybridization.3.In vitro experiments: Normal prostate epithelial cells and prostate cancer cells were cultured in a suitable environment.CircPRKD3 expression in RWPE-1 cells and prostate cancer cells was detected by RT-PCR and immunofluorescence in situ hybridization.4.PC3 cells were further tested as representative of prostate cancer cells.PC3 cells were cultured in vitro and circPRKD3 was over-expressed in vitro.The effects of circPRKD3 were detected by real-time quantitative PCR,Western blot,Transwell and wound healing experiments.5.The design can lower expression of circPRKD3 and low expression of circPRKD3 in prostate cancer cells.Subsequently,quantitative PCR,Western blot experiments,Transwell experiments,and wound healing experiments were used to verify the effect of circPRKD3.Results:1.Detection of differential circular RNA in prostate cancer and benign prostatic hyperplasia tissues.Three cases of human prostate cancer tissues and their matched pairs of BPH tissues were randomly selected.A total of 15 different circRNAs were found in these specimens,and a larger circPRKD3 was selected as the study object.2.CircPRKD3 is low in prostate cancer tissue.Real-time quantitative PCR and FISH experiments confirmed that the expression level of circPRKD3 in prostate cancer tissues was significantly lower than that in benign prostatic hyperplasia tissues.3.CircPRKD3 is low in prostate cancer cellsThe expression level of circPRKD3 in prostate cancer cells was lower than that in prostate epithelial cells,and PC3 cells showed the most significant difference,and PC3 cells were the representative of further experiments.Immunofluorescence experiments showed that circPRKD3 was down-regulated in prostate cancer cells.Taken together,the results indicate that the expression level of circPRKD3 in prostate cancer cells is significantly downregulated.4.Overexpression of circPRKD3 inhibited the proliferation and migration of prostate cancer cells in vitro.PCR,Transwell experiments,wound healing experiments,and Western Blot experiments showed that overexpression of circPRKD3 could inhibit the proliferation and migration of prostate cancer cells in vitro.5.Knockdown circPRKD3 can promote the proliferation and migration of prostate cancer cells in vitro.PCR,Transwell experiments,wound healing experiments,and Western Blot experiments showed that knocking down circPRKD3 can promote the proliferation and migration of prostate cancer cells in vitro.Conclusions:1.CircPRKD3 is present in normal prostate tissue,which is downregulated in prostate cancer tissue.2.CircPRKD3 is a gene that is involved in the proliferation and migration of prostate cancer cells.3.Overexpression of circPRKD3 can inhibit the proliferation and migration of prostate cancer cells.4.Knockdown circPRKD3 can promote the proliferation and migration of prostate cancer cells.