Study On The Therapeutic Mechanism Of Sanhuang Ningjiao For The Treatment Of Grade ? Moderate Inflammatory Acne Vulgaris And Reduction Of Acne Scar

Objective: To observe the clinical effect of Sanhuang Ningjiao in the treatment of class ? moderate inflammatory acne vulgaris,and the intervention mechanism for reducing acne scar of sanhuang ningjiao,explore the specific mechanism of action,and provide a theoretical basis for clinical use.Methods:clinical observation: 60 cases of moderate inflammatory acne vulgaris patients were randomly divided into treatment group and control group,the treatment group were treated by Sanhuang ningjiao,the control group was treated by external use of vitamin A cream,once a day,for 4 weeks.Every 2 weeks,the patients' skin lesions,skin lesions and pain scores and the main curative effect were recorded.After 4 weeks of treatment,the total effective rate was calculated.The secondary skin lesions such as amount of scar,pigmentation,and dark erythema of two groups of treatment after 4weeks were also observed.Experimental study: a model of acne was established by using 100% oleic acid evenly on the bilateral inner ear ducts of the rat.They were randomly divided into six groups by weight: control group,model group,vitamin A acid cream positive control group,Sanhuang ningjiao high dose group,Sanhuang ningjiao medium dose group,low dose group of Sanhuang ningjiao.After the treatment,the auricle of the rats in each group was cut and the histopathological changes were observed.The content of TLR2 and TLR4 in the serum of rats was detected by ELISA.Result:Clinical observation:1.treatment for 2 weeks,the scores of skin lesions and skin pain in the two groups were lower than those before treatment,and the difference was statistically significant(P<0.05).2.treatment for 4 weeks,the scores of all the symptoms were decreased compared with those before the treatment,and the difference was statistically significant (P<0.05).3.treatment for 2 weeks,the two groups of effects in the amount of skin lesion and pain contrast no difference.For 4 weeks,Sanhuang ningjiao group was more effective than vitamin A acid cream group.4.for 2 weeks,4 weeks,Comparison of curative effect of two groups shows that Sanhuang ningjiao group was better than vitamin A acid cream group.5.4 weeks of treatment,comparison of the effect of reducing secondary skin lesions shows that Sanhuang ningjiao group was better than vitamin A acid cream group(P<0.05).6.4 weeks after the treatment,Sanhuang ningjiao group total effective rate was96.67%,vitamin A acid cream group total effective rate was 80%,there were significant differences between the two groups(P<0.05).Animal studies:The content of TLR2 and TLR4 in the serum of rats was detected.Compared with the model group,Sanhuang ningjiao high dose group and vitamin A acid cream group TLR2 and TLR4 were decreased(P<0.01);and there is a dose-response relationship.Conclusion: the overall effect of Sanhuang ningjiao in grade ? moderate inflammatory acne treatment is better than vitamin A acid cream.Sanhuang ningjiao has better effect in the improvement of lesion counts and skinswollen pain.Sanhuang ningjiao group has better effect in the reduction of acne secondary lesions such as dark erythema,pigmentation,acne scar.The mechanism may be related to decreasing the content of TLR2 and TLR4 in serum,alleviating the excessive inflammatory reaction,controlling the development of skin lesions to pustules,cysts,nodules and so on,and inhibiting the infiltration of inflammatory cells,thereby reducing the formation of acne scar with inflammatory skin lesions.