A Correlation Study Of HR-HPV Load And Prognosis Of Cervical Lesions

Object: Using HC-II,short of the second generation hybridization capture experiment,as a method to calculate,to explore the correlation of high-risk human papillomavirus(HR-HPV)load and cervical lesions of different grades and its clinical value in predicting postoperative residual and recurrence of cervical lesions.Methods: The method of retrospective analysis was used.Patients with HPV positive were selected from January 2012 to December 2016 in the first affiliated Hospital of Shi he zi University Medical College.According to the histopathological results as the diagnostic criteria,follow up patients' condition,and analyze the correlation between the HPV viral load and the prognosis of cervical lesions according to the comparison of the examination results and the final pathological diagnosis.To investigate the relationship between high risk HPV-DNA load,cervical lesions and cervical cancer and its clinical value in predicting postoperative residual and recurrent lesions.Results:1.Analysing HPV load groups and cervical lesions before treatment,as P=0.000,we can found that cervical lesions were higher when the cervical lesions got more seriously.Compared the cervical lesions of HSIL and above with the load group of HR-HPV,the results showed that OR=0.275 and 95% CI was 0.159-0.475 when HPV load was below 1000,which suggests that the HPV load groups below the high grade had a positive correlation with the cervical lesions before treatment.2.They had no correlation as P =0.156 in the analysis of HPV load groups and age groups before treatment,while P<0.05 when the age grouped by 50.Then in the bifid comparison among groups,the difference between age group of 36-50 years and over 50 years and HPV load groups of medium and high load has a statistical significance,meaning people over 50 years old had a higher risk of 1000 and above of HPV load than younger women.3.There were significant differences in the persistent infection rate among different grades of cervical lesions and age groups(P<0.05).Compared the age group of over50 with the group of below or equal to 35,the result is P=0.02,OR=0.42,and 95%CI was 0.203-0.871,suggesting that the persistent infection rate of HR-HPV in HSIL is the highest,and in cervical precancerous lesions,it is related to the degree of cervical lesions,which means the persistent infection rate was higher in patients over 50 years old when they grouped by ages.4.Compared the age groups with cervical lesions,the results showed that P=0.04,which suggests that older patients are prone to higher grade cervical lesions.5.Computing the negative rate of each pathological group comes to a conclusion that the degree of cervical lesion and the rate of negative conversion were basically related after treatment.And the average rate of negative conversion increased with time within 1 year after treatment,while decreased in people who turned negative after 2years.Using single factor ANOVA analysis,the result of P>0.05 respects that the time of turning negative for cervical lesion groups has no significant difference,making an suggestion that the group of cervical lesions is not related to the time of turning negative.6.Computing the negative rate of each HPV load group comes to a conclusion that The HR-HPV load group was correlated with the rate of negative conversion.In another word,the rate of turning negative increased with time within 1 year after operation,and decreased in the group of people who turned negative after 2 years.There is no correlation in comparison of negative conversion rate among different loads,because sample size is different.Using single factor ANOVA analysis,the result of P>0.05 respects that the time of turning negative for cervical lesion groups has no significant difference,making an suggestion that the amount of HPV before treatment is not related to the time of turning negative.7.Computing the negative rate of each age group comes to a conclusion that the negative rate of 35-50 years old was the highest after treatment of one year,and there was a positive correlation between age and negative rate when they had at least 1 year treatment.Using single factor ANOVA analysis,it has statistical differences among the age group and the time of turning negative,as the younger the age,the shorter the time of turning negative.8.Repeated measurement analysis of HPV load before and after treatment suggests that difference was statistically significant.We can make an conclusion that HPV decreased after treatment within half a year,and all kinds of treatment could effectively reduce the HPV load,but the HPV load increased after one year of treatment,and the HPV load of 2 years after treatment was higher than that after one year.Conclusion: Preoperative high HR-HPV load possibly was an independent risk factor for persistent HPV infection after operation.The degree of cervical lesions before treatment was related to the HR-HPV load group,but not to the HPV load.The degree of cervical lesion and age may be related factors of HR-HPV negative conversion.