The Relationship Between CXCL12 Gene Polymorphism And Large Atherosclerotic Cerebral Infarction

Objective:To investigate the impact of CXCL12 gene polymorphisms and on the risk of large artery atherosclerotic(LAA)stroke and the distribution of cerebralvascular stenosis.We further investigate the association between CXCL12 gene-smoking interactions and the risk of LAA stroke.Methods:A total of 290 patients with cerebralvascular disease and 310 normal controls were collected,who from Department of Neurology,Weifang Yi Du Center Hospital in October 2015 to October 2017.According to the distribution of atherosclerotic stenosis,the patients were divided into three groups,including intracranial angiostenosis(ICAS)group extracranial angiostenosis(ECAS)group,and intracranial-extracranial angiostenosis(IC-ECAS)group.The methods of polymerase chain reaction-restriction fragment length polymorphism,combined with DNA sequencing,were used to detect the CXCL12 rs1801157.Hardy-Weinberg equilibrium(HWE)was assessed using chi-aquare test.Pearson'X~2 test or Fisher'exact test were applied to compare the frequencies of different genotypes and alleles in CXCL12 gene.Logistic regression was applied to analyze associations between CXCL12 and LAA stroke.Cross analysis of additive model was used to evaluate gene-smoking interactions.Results:1.The genotype AA+GG and allele A of rs1801157 in case group were in higher frequency than that in controls(13.4%vs 2.8%,OR=3.056,95%CI1.646-5.674,P<0.001;10.2%vs3.7%,OR=2.939,95%CI1.790-4.827,P<0.001).Multivariate logistic regression analysis showed that AA/AG genotype was a risk factor for LAA stroke(OR=1.990,95%CI=1.334-2.968,P=0.015).What's more,a significant increased risk for LAA stroke was observed for patients incigarettesmokingstatus(OR=2.018,95%CI=1.492-2.723,P<0.001).Additionally,hypertension,diabetes and BMI are independent risk factors for LAAstroke as well.2.The incidence of ICAS and IC-ECAS in LAA stroke patients were 35.86%and 39.65%respectively,being obviously higher than the incidence of ECAS(24.48%).3.Additive model analysis showed significant interactions between rs1801157 and smoking(RERI=3.545,95%CI=1.303-9.631).Smokers with A allele of rs1801157 showed significantly increased risk of LAAstroke(OR=5.586,95%CI=2.263-13.791,P<0.001).Conclusions:1.The Aallele of rs1801157 within CXCL12 may be a risk factor of LAAstroke.2.The interactions exsit between rs1801157 and smoking,which can significantly increased the risk of LAAstroke.3.The rs1801157 polymorphisms in CXCL12 gene were not associated with the distribution of atherosclerotic stenosis in LAAstroke patients.