Effect Of EB Virus Infection On Helicobacter Pylori Infection And Expression Of Tumor Associated Proteins In Gastric Cancer

Objective:Gastric cancer is a common malignant tumor.Many carcinogenic factors and a variety of tumor associated genes are involved in the occurrence of gastric cancer.EB virus positive gastric cancer is a subtype of gastric cancer resulting from the clonogenic growth of the gastric epithelial cells infected by EB virus.The molecular characteristics of EB virus positive gastric cancer are not clear.This study aims to analyze the expression of tumor associated proteins in this type of gastric cancer,and to explore the relationship between the occurrence and development of EB positive gastric cancer and the expression of the above factors.Methods:In situ hybridization was used to detect the infection of EB virus in gastric cancer specimens.Immunohistochemical method was used to detect the expression of tumor associated proteins(CEA,C-erbB-2,EGFR,Ki-67,P53,CK7,AFP,CD56,CD31,Syn and CgA).The status of Helicobacter pylori(HP)was detected by histopathological staining methods.Measurement data were compared using independent sample t test.Count data were compared usingc~2 test and Fisher's exact test.P<0.05 was considered to be statistically significant.Results:1.Of the 58 cases with gastric cancer,5 cases were EB virus positive,and the positive rate was 8.6%.EB virus positive gastric cancer was more common in men,and there was a statistically significant difference between sex(c~2=4.766,P<0.05).There was no significant difference in age,degree of differentiation and location of cancer between EB virus positive and negative gastric cancer.2.The positive rate of C-erbB-2 in EB virus positive gastric cancer was 80%,higher than that in negative gastric cancer(28.3%),and the difference was statistically significant(P<0.05).The positive rate of CgA in EB virus positive gastric cancer was60%,higher than that in negative gastric cancer(15.1%),and the difference was statistically significant(P<0.05).There was no significant difference in the expression of CEA,EGFR,Ki-67,P53,CK7,AFP,CD56,CD31 and Syn between EB virus positive and negative gastric cancer.3.The positive rate of HP infection in EB positive gastric cancer was 80.0%(4/5),and that in EB negative gastric cancer was 58.5%(31/53).The difference was not statistically significant(?~2=0.21,P=0.64)?Conclusions:1.EB virus positive gastric cancer occurs predominantly in men.2.The expression rate of C-erbB-2 and CgA in EB virus positive gastric cancer is higher than that in EB virus negative gastric cancer.It is suggested that EB virus infection may increase the expression of these two types of tumor associated proteins,thus promoting the occurrence and development of gastric cancer.However,the mechanism is not yet clear.3.Both EB virus and HP play a role in the occurrence and development of gastric cancer,but there is no interaction between them.