The Protective Effects Of Collagen Peptides From Walleye Pollock Skin (CPWPS) Against Ovariectomized Rat Osteoporosis

Objective:To explore the feasibility of preventing and treating osteoporosis by investigating the effect of collagen peptides from walleye pollock skin(CPWPS)on the microstructure of osteoporosis model in ovariectomized rats.Methods:Sixty adult Wistar female rats,weighing(250±10)g,were randomly divided into five groups(12 rats each group):normal group,osteoporosis model group,CPWPS prevention group,CPWPS dose group?,and CPWPS dose group?.In addition to the normal group,the other groups were used to remove rat ovariectomized osteoporosis model.CPWPS prevention group begans 4 weeks before surgery,CPWPS dose group?,CPWPS dose group? from 6 weeks after the start,according to the daily 1.0,0.5,1.0g/kg CPWPS gavage for 6 weeks;and the rats in CPWPS dose group?and CPWPS dose group?were treated with stomach perfusion of the CPWPS(0.5,1.0 g/kg respectively)at 6 weeks after operation for 6 weeks.The rats in normal group and osteoporosis model group were given equal volume of normal saline at the same time after operation.At 24 hours after the last administration,the femoral gray value of rats in groups normal group and osteoporosis model group were measured by X-ray film;HE staining was performed on the proximal tibial bone of the left side in 4 groups;the histopathological changes of the bone were observed and the trabecular number(TN),mean trabecular plate thickness(MTPT),mean trabecular plate spacing(MTPS),trabecular bone volume(TBV),mean bone cortical thickness(MBCT)were measured;immunohistochemical staining was performed to observe the expression levels of caltitonin receptor(CTR)and interleukin 1(IL-1).Results:The femoral gray value of osteoporosis model group was significantly lower than that of normal group(t=45.130,P=0.000),which indicated that the ovariectomized rat model was successfully prepared.Histological observation showed that TN,MTPS,TBV,and MBCT in normal group,CPWPS prevention group,and CPWPS dose group?were significantly different from those in osteoporosis model group(P<0.05).The histological parameters of bone tissue in CPWPS prevention group were significantly different from those in CPWPS dose group?and CPWPS dose group?(P<0.05).TN,MTPS ? TBV,and MBCT in CPWPS dose group?were significantly different from those in normal group(P<0.05);only MTPS in CPWPS dose group?was significantly different from that in normal group(P<0.05).MTPS,TBV,and MBCT in CPWPS dose group?were significantly different from those in CPWPS dose group?(P<0.05).The immunohistochemical staining showed that the levels of CTR and IL-1 in groups normal group,CPWPS prevention group,CPWPS dose group?,and CPWPS dose group? were lower than those in osteoporosis model group,in CPWPS prevention group and CPWPS dose group?were lower than in CPWPS dose group?,showing significant differences(P<0.05).Conclusion:Collagen peptides from walleye pollock skin can improve the bone microstructure of osteoporotic rats,and its mechanism may be related to the inhibition of CTR and IL-1 expression in bone tissue,but it has not been found to prevent osteoporosis.