Effects Of Collagen Peptides From Walleye Pollock On Biological Characteristics Of Human Skin Fibroblasts

Objective: To observe the effect of collagen fillet peptides on the biological characteristics of human skin fibroblasts cultured in vitro.Methods: Human skin fibroblasts were cultured in vitro.Different concentrations of collagen peptides were added into the culture medium and divided into experimental control group and normal control group.The effect of collagen peptides on the proliferation of fibroblasts was detected by enzyme method.Annexin V/PI double color method was used to detect the effect of collagen peptide on fibroblasts.The expression of TGF-?1and SOD that was effected by Collagen peptides was detected by Elisa kit.The effect of collagen peptides on the synthesis of type I collagen in human skin fibroblasts was detected by western blot.The effect of collagen peptides on the migration of human skin fibroblasts was detected by scratch test.Results: 1.The proliferation of human skin fibroblasts was examined by CCK-8 kit after 48 hours of treatment with human fibroblasts at different concentrations of collagen peptides.The results showed that the proliferation rate of fibroblasts showed a dose-dependent effect after stimulation with collagen peptides.With the increase of collagen peptides concentration,the proliferation rate of the cells was also increased,which was significantly different from that of the blank control group(P<0.05).The The proliferation rate of fibroblasts was the fastest when the concentration of collagen peptides was 25mg/ml.2.Cell apoptosis: cells were treated with Annexin-v-FITC/PI kit for 25 min/ml collagen peptide treatment for 24 h,and the effect of collagen peptide on human skin fibroblast apoptosis was examined.Compared with the blank control group,the apoptotic ratio of fibroblasts was significantly decreased(P <0.05).Collagen peptides could effectively promote the proliferation of human skin fibroblasts and inhibit the apoptosis of collagen cells,Fibroblasts secrete TGF-?1,SOD and other active factors(t=4.07,F=774.23,2 100.63,P<0.05).Compared with the blank control group,the migration ability of collagen peptides group was enhanced(P<0.05).3.Elisa results: After stimulation with different concentrations of collagen peptides,the results showed that TGF-?1 and SOD expression were increased in a dose-dependent manner,25mg/ml and50mg/ml concentrations were significantly more significant than the blank control group(P<0.01),of which the most significant effect was 50mg/ml.4.Scratch test: The results showed that the cell migration rate after collagen peptides was significantly increased(P<0.01).5.Western blot: The results showed that the expression of type I collagen in human skin fibroblasts after collagen peptides was increased(P<0.05).Conclusion:Collagen peptides has a good role in promoting cell proliferation,inhibiting cell apoptosis,enhancing cell migration ability and antioxidant protection,and can promote type I collagen synthesis,which may play an important role in wound healing.