| Objective: nephrotic syndrome is a group of clinical syndromes caused by a variety of causes of increased glomerular basement membrane permeability,resulting in the loss of large quantities of protein from the urine.The clinical features arehigh-grade proteinuria,hypoalbuminemia,hyperlipidemia,and obvious edema.Nephrotic syndrome is one of the most common pediatric kidney disease,clinically and atopic disease complicated with some children,IgE increased significantly,on children with nephrotic syndrome blood IgE,blood and urinary leukotriene changes has great guiding significance in the pathogenesis of nephrotic syndrome in children treated for this kind of disease.Methods: This is a single center,prospective randomized controlled clinical research methods,selected from the Shengjing Hospital affiliated to the China Medical University pediatric kidney rheumatology ward diagnosed with primary nephrotic syndrome in children,clinical data were collected and auxiliary examination results(age,gender,IgE,24 hour urine protein,after treatment,remission time,blood eosinophil cell count,number of recurrence within 1 years after treatment).The fasting venous blood of the children in the group was left 2-4ml,and the middle part of the urine 10 ml was cleaned in the morning.After centrifugation,it was kept in the refrigerator at-40 centigrade to be tested.The serum leukotriene B4 and urinary leukotriene E4 levels in 33 cases of IgE high nephrotic syndrome were measured by enzyme-linked immunosorbent assay(ELISA),and compared with the levels of leukotriene B4 and urinary leukotriene E4 in 22 cases of IgE normal nephrotic syndrome.The levels of serum IgE,leukotrienes B4 and urinary leukotrienes E4 in the children were compared with the 24 hour urine protein quantitative and remission time.Results: A total of 55 hospitalized children with nephrotic syndrome were selected from October2015 to December 2016 in pediatric nephrology department of Shengjing Hospital.They were divided into IgE normal group and IgE high group.There were 22 cases in the normal IgE group(control group),of which 13 were male and 9 in women.The age range was 6±5 years and the average age was 3.55 years old.The high group of IgE(experimental group)was 33,of which 24 were male and 9 women,the age range was 7±6 years,the average age was 4.52 years old.The two groups were all in accordance with the diagnostic criteria of nephrotic syndrome.There was no corticosteroid treatment before entering the group,and there was no significant difference in age and sex.Leukotriene B4 concentration of blood test(75.53±67.19)pg/ml,urinary leukotriene concentration(742.51±668.69)pg/ml,IgE level(1552.6±1447.4)IU/mL control group;blood concentration of leukotriene B4(71.75±67.36)pg/ml,urinary leukotriene concentration(899.99±733.91)pg/ml,IgE(level 46.9±44.6)IU/mL;IgE group is higher in children with idiopathic nephrotic syndrome and urinary leukotriene B4 and leukotriene IgE normal group nephropathy between syndrome serum leukotriene B4 and urinary leukotriene E4 were not statistically significant.In all cases,the level of serum IgE and urine protein in 24 hours after treatment,remission time and recurrence within 1 years the number of statistically significant correlation;serum leukotriene B4 and urinary leukotriene E4 concentration and serum IgE,urine protein in 24 hours after treatment,remission time,blood eosinophil the count and the frequency of recurrence within 1 year showed no statistical correlation.Conclusion: the level of serum IgE and urine protein in 24 hours,after treatment,the remission time and the frequency of recurrence within 1 year,one of the index can be used to monitor the condition and effect of blood;leukotriene B4 and urinary leukotriene E4 level and serum IgE level,24 hour urinary protein and remission were no correlation between.Whether the levels of leukotriene B4 and E4 are involved in the occurrence and development of the disease of children with nephrotic syndrome should be further explored. |
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